A new microbial electrode for BOD estimation

Isao Karube*, Tadashi Matsunaga, Shuichi Suzuki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)


A new microbial electrode using immobilized Clostridium butyricum was prepared for biochemical oxygen demand (BOD) estimation of wastewaters. The current of the electrode was decreased with time until a steady state was reached. The steady state current was in all cases attained within 30-40 min at 37°C, and the maximum current output was obtained at 37°C and pH between 6.2 and 7.0. A linear relationship was obtained between the steady state current and BOD. The steady state current values were reproducible within ±7% of the relative error. The BOD of industrial wastewaters can be estimated by using the microbial electrode. Relative error of the BOD estimation of industrial wastewaters was within ± 10%. The current output of the microbial electrode was almost constant for 30 days.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalJournal of Solid-Phase Biochemistry
Issue number2
Publication statusPublished - 1977 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Biochemistry


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