A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening

Mira Syahfriena Amir Rawa; Toshihiko Nogawa; Akiko Okano; Yushi Futamura; Takemichi Nakamura; Habibah A. Wahab; Hiroyuki Osada

doi:10.1093/bbb/zbaa051

A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening

Mira Syahfriena Amir Rawa, Toshihiko Nogawa, Akiko Okano, Yushi Futamura, Takemichi Nakamura, Habibah A. Wahab, Hiroyuki Osada^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A new peptaibol, RK-026A (1) was isolated from a fungus, Trichoderma sp. RK10-F026, along with atroviridin B (2), alamethicin II (3), and polysporin B (4) as a cytotoxic compound, which was selected by principal component analysis of the MS data from 5 different culture conditions. The structure of 1 was determined as a new atroviridin B derivative containing Glu at the 18th residue instead of Gln by NMR and HR-MS analyses including the investigation of detailed MS/MS fragmentations. 1 showed cytotoxicity toward K562 leukemia cells at an IC50 value of 4.1 mm.

Original language	English
Pages (from-to)	69-76
Number of pages	8
Journal	Bioscience, Biotechnology and Biochemistry
Volume	85
Issue number	1
DOIs	https://doi.org/10.1093/bbb/zbaa051
Publication status	Published - 2021 Jan 1
Externally published	Yes

Keywords

K562
cytotoxicity
fungi
natural product
peptaibols

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1093/bbb/zbaa051

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title = "A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening",

abstract = "A new peptaibol, RK-026A (1) was isolated from a fungus, Trichoderma sp. RK10-F026, along with atroviridin B (2), alamethicin II (3), and polysporin B (4) as a cytotoxic compound, which was selected by principal component analysis of the MS data from 5 different culture conditions. The structure of 1 was determined as a new atroviridin B derivative containing Glu at the 18th residue instead of Gln by NMR and HR-MS analyses including the investigation of detailed MS/MS fragmentations. 1 showed cytotoxicity toward K562 leukemia cells at an IC50 value of 4.1 mm.",

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author = "Rawa, {Mira Syahfriena Amir} and Toshihiko Nogawa and Akiko Okano and Yushi Futamura and Takemichi Nakamura and Wahab, {Habibah A.} and Hiroyuki Osada",

note = "Funding Information: This work was supported in part by JSPS KAKENHI under Grant [numbers JP17H06412 and JP20K05858]. Publisher Copyright: {\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.",

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doi = "10.1093/bbb/zbaa051",

language = "English",

volume = "85",

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AU - Rawa, Mira Syahfriena Amir

AU - Nogawa, Toshihiko

AU - Okano, Akiko

AU - Futamura, Yushi

AU - Nakamura, Takemichi

AU - Wahab, Habibah A.

AU - Osada, Hiroyuki

N1 - Funding Information: This work was supported in part by JSPS KAKENHI under Grant [numbers JP17H06412 and JP20K05858]. Publisher Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

PY - 2021/1/1

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AB - A new peptaibol, RK-026A (1) was isolated from a fungus, Trichoderma sp. RK10-F026, along with atroviridin B (2), alamethicin II (3), and polysporin B (4) as a cytotoxic compound, which was selected by principal component analysis of the MS data from 5 different culture conditions. The structure of 1 was determined as a new atroviridin B derivative containing Glu at the 18th residue instead of Gln by NMR and HR-MS analyses including the investigation of detailed MS/MS fragmentations. 1 showed cytotoxicity toward K562 leukemia cells at an IC50 value of 4.1 mm.

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KW - cytotoxicity

KW - fungi

KW - natural product

KW - peptaibols

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A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening

Abstract

Keywords

ASJC Scopus subject areas

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