A new relaxin-like gonad-stimulating peptide identified in the starfish Asterias amurensis

Masatoshi Mita, Misaki Daiya, Shogo Haraguchi, Kazuyoshi Tsutsui, Yoshitaka Nagahama

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Relaxin-like gonad-stimulating peptide (RGP) of starfish Asterina pectinifera was the first invertebrate gonadotropin to have its chemical structure identified. However, it is unclear whether gonadotropic hormones in other species starfish are relaxin-like peptides. Thus, this study tried to identify the molecular structure of gonadotropic hormone in Asterias amurensis. As a result, we identified A. amurensis gonadotropic hormone as the RGP (AamRGP). The DNA sequence encoding AamRGP consisted of 330 base pairs with an open reading frame encoding a peptide of 109 amino acids (aa), including a signal peptide (26 aa), B-chain (20 aa), C-peptide (38 aa) and A-chain (25 aa). Comparing with A. pectinifera RGP (ApeRGP), the amino acid identity levels between AmaRGP and ApeRGP were 58% for the A-chain and 73% for the B-chain. Furthermore, chemical synthetic AamRGP induced gamete spawning and oocyte maturation in ovarian fragments of A. amurensis. In contrast, the ovary of A. pectinifera failed to respond to the AamRGP. This suggested that AamRGP is a new relaxin-like peptide.

    Original languageEnglish
    Pages (from-to)144-149
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume222
    DOIs
    Publication statusPublished - 2015 Oct 1

    Fingerprint

    Asterias
    relaxin
    Starfish
    Relaxin
    Asteroidea
    Asterina
    Gonads
    Asterina pectinifera
    gonads
    peptides
    Amino Acids
    Peptides
    amino acids
    Hormones
    hormones
    chemical structure
    C-Peptide
    Invertebrates
    Protein Sorting Signals
    Molecular Structure

    Keywords

    • Coding-DNA sequence
    • Gamete spawning
    • Relaxin-like gonad-stimulating peptide
    • Species-specificity
    • Starfish Asterias amurensis

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    A new relaxin-like gonad-stimulating peptide identified in the starfish Asterias amurensis. / Mita, Masatoshi; Daiya, Misaki; Haraguchi, Shogo; Tsutsui, Kazuyoshi; Nagahama, Yoshitaka.

    In: General and Comparative Endocrinology, Vol. 222, 01.10.2015, p. 144-149.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Daiya, Misaki ; Haraguchi, Shogo ; Tsutsui, Kazuyoshi ; Nagahama, Yoshitaka. / A new relaxin-like gonad-stimulating peptide identified in the starfish Asterias amurensis. In: General and Comparative Endocrinology. 2015 ; Vol. 222. pp. 144-149.
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