A new simple method to introduce marker-free deletions in the Bacillus subtilis genome

Takuya Morimoto; Katsutoshi Ara; Katsuya Ozaki; Naotake Ogasawara

doi:10.1266/ggs.84.315

A new simple method to introduce marker-free deletions in the Bacillus subtilis genome

Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara

Research output: Contribution to journal › Article

29 Citations (Scopus)

Abstract

A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible.

Original language	English
Pages (from-to)	315-318
Number of pages	4
Journal	Genes and Genetic Systems
Volume	84
Issue number	4
DOIs	https://doi.org/10.1266/ggs.84.315
Publication status	Published - 2009 Dec 1
Externally published	Yes

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Keywords

Bacillus subtilis
Marker-free mutant
mazF

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Morimoto, T., Ara, K., Ozaki, K., & Ogasawara, N. (2009). A new simple method to introduce marker-free deletions in the Bacillus subtilis genome. Genes and Genetic Systems, 84(4), 315-318. https://doi.org/10.1266/ggs.84.315

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Access to Document

10.1266/ggs.84.315