Abstract
A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible.
Original language | English |
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Pages (from-to) | 315-318 |
Number of pages | 4 |
Journal | Genes and Genetic Systems |
Volume | 84 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |
Keywords
- Bacillus subtilis
- Marker-free mutant
- mazF
ASJC Scopus subject areas
- Molecular Biology
- Genetics