A new transcription element in the JC virus enhancer

T. Tamura, A. Aoyama, Takafumi Inoue, M. Miura, M. Mikoshiba

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The enhancer of JC virus restricts its gene expression to the brain. In a previous study, we demonstrated an enhancer element, nuclear factor I (NFI) motif, in the middle of the enhancer. Here, we demonstrate that the NFI motif is a tissue-specific element. We further present a new tissue-specific element, SacI motif, just upstream from the NFI motif. These motifs showed transcriptional enhancement both in vitro and in vivo and acted upon a heterologous adenovirus major late promoter. DNase I footprint analyses demonstrated that the SacI motif bound to a brain nuclear factor, and that its binding region overlapped with the NFI motif. Gel shift experiments with the SacI motif revealed that the populations of SacI motif factors in the brain and HeLa cell extracts were different. Together with our previous findings about tissue-specific NFI-like factor(s), cooperation of NFI motif and SacI motif factors may be required for the strong brain specificity of the viral gene expression.

Original languageEnglish
Pages (from-to)1829-1833
Number of pages5
JournalJournal of General Virology
Volume71
Issue number8
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

NFI Transcription Factors
JC Virus
Brain
Gene Expression
Viral Genes
Deoxyribonuclease I
Cell Extracts
HeLa Cells
Adenoviridae
Gels
Population

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Tamura, T., Aoyama, A., Inoue, T., Miura, M., & Mikoshiba, M. (1990). A new transcription element in the JC virus enhancer. Journal of General Virology, 71(8), 1829-1833.

A new transcription element in the JC virus enhancer. / Tamura, T.; Aoyama, A.; Inoue, Takafumi; Miura, M.; Mikoshiba, M.

In: Journal of General Virology, Vol. 71, No. 8, 1990, p. 1829-1833.

Research output: Contribution to journalArticle

Tamura, T, Aoyama, A, Inoue, T, Miura, M & Mikoshiba, M 1990, 'A new transcription element in the JC virus enhancer', Journal of General Virology, vol. 71, no. 8, pp. 1829-1833.
Tamura T, Aoyama A, Inoue T, Miura M, Mikoshiba M. A new transcription element in the JC virus enhancer. Journal of General Virology. 1990;71(8):1829-1833.
Tamura, T. ; Aoyama, A. ; Inoue, Takafumi ; Miura, M. ; Mikoshiba, M. / A new transcription element in the JC virus enhancer. In: Journal of General Virology. 1990 ; Vol. 71, No. 8. pp. 1829-1833.
@article{b3ede4a113de42a589fc6a7351ba4cfe,
title = "A new transcription element in the JC virus enhancer",
abstract = "The enhancer of JC virus restricts its gene expression to the brain. In a previous study, we demonstrated an enhancer element, nuclear factor I (NFI) motif, in the middle of the enhancer. Here, we demonstrate that the NFI motif is a tissue-specific element. We further present a new tissue-specific element, SacI motif, just upstream from the NFI motif. These motifs showed transcriptional enhancement both in vitro and in vivo and acted upon a heterologous adenovirus major late promoter. DNase I footprint analyses demonstrated that the SacI motif bound to a brain nuclear factor, and that its binding region overlapped with the NFI motif. Gel shift experiments with the SacI motif revealed that the populations of SacI motif factors in the brain and HeLa cell extracts were different. Together with our previous findings about tissue-specific NFI-like factor(s), cooperation of NFI motif and SacI motif factors may be required for the strong brain specificity of the viral gene expression.",
author = "T. Tamura and A. Aoyama and Takafumi Inoue and M. Miura and M. Mikoshiba",
year = "1990",
language = "English",
volume = "71",
pages = "1829--1833",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "8",

}

TY - JOUR

T1 - A new transcription element in the JC virus enhancer

AU - Tamura, T.

AU - Aoyama, A.

AU - Inoue, Takafumi

AU - Miura, M.

AU - Mikoshiba, M.

PY - 1990

Y1 - 1990

N2 - The enhancer of JC virus restricts its gene expression to the brain. In a previous study, we demonstrated an enhancer element, nuclear factor I (NFI) motif, in the middle of the enhancer. Here, we demonstrate that the NFI motif is a tissue-specific element. We further present a new tissue-specific element, SacI motif, just upstream from the NFI motif. These motifs showed transcriptional enhancement both in vitro and in vivo and acted upon a heterologous adenovirus major late promoter. DNase I footprint analyses demonstrated that the SacI motif bound to a brain nuclear factor, and that its binding region overlapped with the NFI motif. Gel shift experiments with the SacI motif revealed that the populations of SacI motif factors in the brain and HeLa cell extracts were different. Together with our previous findings about tissue-specific NFI-like factor(s), cooperation of NFI motif and SacI motif factors may be required for the strong brain specificity of the viral gene expression.

AB - The enhancer of JC virus restricts its gene expression to the brain. In a previous study, we demonstrated an enhancer element, nuclear factor I (NFI) motif, in the middle of the enhancer. Here, we demonstrate that the NFI motif is a tissue-specific element. We further present a new tissue-specific element, SacI motif, just upstream from the NFI motif. These motifs showed transcriptional enhancement both in vitro and in vivo and acted upon a heterologous adenovirus major late promoter. DNase I footprint analyses demonstrated that the SacI motif bound to a brain nuclear factor, and that its binding region overlapped with the NFI motif. Gel shift experiments with the SacI motif revealed that the populations of SacI motif factors in the brain and HeLa cell extracts were different. Together with our previous findings about tissue-specific NFI-like factor(s), cooperation of NFI motif and SacI motif factors may be required for the strong brain specificity of the viral gene expression.

UR - http://www.scopus.com/inward/record.url?scp=0025080483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025080483&partnerID=8YFLogxK

M3 - Article

C2 - 2167938

AN - SCOPUS:0025080483

VL - 71

SP - 1829

EP - 1833

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 8

ER -