A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer

Hideyuki Terazono, Hyonchol Kim, Masahito Hayashi, Akihiro Hattori, Fumimasa Nomura, Tomoyuki Kaneko, Kenji Yasuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.

Original languageEnglish
Article numbere42485
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - 2012 Aug 3
Externally publishedYes

Fingerprint

alginates
Sorting
Cardiac Myocytes
sorting
Neurons
neurons
Cells
embryonic stem cells
Stem cells
cultured cells
Cultured Cells
neurites
nondestructive methods
chelates
Neurites
cells
functional properties
Buffers
buffers
Transplantation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. / Terazono, Hideyuki; Kim, Hyonchol; Hayashi, Masahito; Hattori, Akihiro; Nomura, Fumimasa; Kaneko, Tomoyuki; Yasuda, Kenji.

In: PLoS One, Vol. 7, No. 8, e42485, 03.08.2012.

Research output: Contribution to journalArticle

Terazono, Hideyuki ; Kim, Hyonchol ; Hayashi, Masahito ; Hattori, Akihiro ; Nomura, Fumimasa ; Kaneko, Tomoyuki ; Yasuda, Kenji. / A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. In: PLoS One. 2012 ; Vol. 7, No. 8.
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