Abstract
A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.
| Original language | English |
|---|---|
| Article number | e42485 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2012 Aug 3 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer. / Terazono, Hideyuki; Kim, Hyonchol; Hayashi, Masahito; Hattori, Akihiro; Nomura, Fumimasa; Kaneko, Tomoyuki; Yasuda, Kenji.
In: PLoS One, Vol. 7, No. 8, e42485, 03.08.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer
AU - Terazono, Hideyuki
AU - Kim, Hyonchol
AU - Hayashi, Masahito
AU - Hattori, Akihiro
AU - Nomura, Fumimasa
AU - Kaneko, Tomoyuki
AU - Yasuda, Kenji
PY - 2012/8/3
Y1 - 2012/8/3
N2 - A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.
AB - A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.
UR - http://www.scopus.com/inward/record.url?scp=84864535440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864535440&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0042485
DO - 10.1371/journal.pone.0042485
M3 - Article
C2 - 22870332
AN - SCOPUS:84864535440
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 8
M1 - e42485
ER -