A Novel Actin Bundling/Filopodium-forming Domain Conserved in Insulin Receptor Tyrosine Kinase Substrate p53 and Missing in Metastasis Protein

Akiko Yamagishi, Michitaka Masuda, Takashi Ohki, Hirofumi Onishi, Naoki Mochizuki

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Insulin receptor tyrosine kinase substrate p53 (IRSp53) has been identified as an SH3 domain-containing adaptor that links Rac1 with a Wiskott-Aldrich syndrome family verprolin-homologous protein 2 (WAVE2) to induce lamellipodia or Cdc42 with Mena to induce filopodia. The recruitment of these SH3-binding partners by IRSp53 is thought to be crucial for F-actin rearrangements. Here, we show that the N-terminal predicted helical stretch of 250 amino acids of IRSp53 is an evolutionarily conserved F-actin bundling domain involved in filopodium formation. Five proteins including IRSp53 and missing in metastasis (MIM) protein share this unique domain and are highly conserved in vertebrates. We named the conserved domain IRSp53/MIM homology domain (IMD). The IMD has domain relatives in invertebrates but does not show obvious homology to any known actin interacting proteins. The IMD alone, derived from either IRSp53 of MIM, induced filopodia in HeLa cells and the formation of tightly packed parallel F-actin bundles in vitro. These results suggest that IRSp53 and MIM belong to a novel actin bundling protein family. Furthermore, we found that filopodium-inducing IMD activity in the full-length IRSp53 was regulated by active Cdc42 and Rac1. The SH3 domain was not necessary for IMD-induced filopodium formation. Our results indicate that IRSp53, when activated by small GTPases, participates in F-actin reorganization not only in an SH3-dependent manner but also in a manner dependent on the activity of the IMD.

Original languageEnglish
Pages (from-to)14929-14936
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number15
DOIs
Publication statusPublished - 2004 Apr 9
Externally publishedYes

Fingerprint

Pseudopodia
Actins
Neoplasm Metastasis
Substrates
Proteins
src Homology Domains
Wiskott-Aldrich Syndrome Protein Family
Amino Acid Receptors
Wiskott-Aldrich Syndrome
Monomeric GTP-Binding Proteins
insulin receptor tyrosine kinase
Insulin Receptor
Invertebrates
HeLa Cells
Vertebrates
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

A Novel Actin Bundling/Filopodium-forming Domain Conserved in Insulin Receptor Tyrosine Kinase Substrate p53 and Missing in Metastasis Protein. / Yamagishi, Akiko; Masuda, Michitaka; Ohki, Takashi; Onishi, Hirofumi; Mochizuki, Naoki.

In: Journal of Biological Chemistry, Vol. 279, No. 15, 09.04.2004, p. 14929-14936.

Research output: Contribution to journalArticle

Yamagishi, Akiko ; Masuda, Michitaka ; Ohki, Takashi ; Onishi, Hirofumi ; Mochizuki, Naoki. / A Novel Actin Bundling/Filopodium-forming Domain Conserved in Insulin Receptor Tyrosine Kinase Substrate p53 and Missing in Metastasis Protein. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 15. pp. 14929-14936.
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