A novel amphibian hypothalamic neuropeptide: Isolation, localization, and biological activity

Aya Koda, Kazuyoshi Ukena, Hitoshi Teranishi, Shinji Ohta, Kazutoshi Yamamoto, Sakae Kikuyama, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Neuropeptides similar to the molluscan cardioexcitatory Phe-Met-Arg-Phe-NH2 have been identified in several vertebrates and characterized by the RFa motif at their C terminus (RFa peptides). In this study, we sought to identify an amphibian hypothalamic RFa peptide that may regulate secretion of hormones by the anterior pituitary gland. An acid extract of bullfrog hypothalami was passed through C-18 reversed-phase cartridges, and then the retained material was subjected to HPLC, initially using a C-18 reversed-phase column. RFa immunoreactivity was measured in the eluted fractions by a dot immunoblot assay employing an antiserum raised against RFa. Immunoreactive fractions were subjected to further cation exchange and reversed-phase HPLC purification. The isolated peptide was a novel RFa peptide and shown to have the sequence Ser-Leu-Lys-Pro-Ala-Ala-Asn-Leu-Pro-Leu-Arg-Phe-NH2. The cell bodies and terminals containing this peptide were localized immunohistochemically in the suprachiasmatic nucleus and median eminence, respectively. This RFa peptide stimulated, in a dose-related way, the release of GH from cultured pituitary cells, its threshold concentration ranging between 10-9 and 10-8 M. This peptide did not have any appreciable effect on the secretion of PRL and gonadotropins. It was ascertained that the peptide was also effective in elevating the circulating GH level when administered systemically. Thus, the amphibian hypothalamus was revealed to contain a novel functional RFa peptide that stimulates GH release. This peptide was designated frog GH-releasing peptide.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalEndocrinology
Volume143
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Amphibians
Neuropeptides
Peptides
Hypothalamus
High Pressure Liquid Chromatography
FMRFamide
Rana catesbeiana
Median Eminence
Suprachiasmatic Nucleus
Anterior Pituitary Gland
Gonadotropins
Anura
Vertebrates
Cations
Immune Sera
Cultured Cells
Hormones
Acids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

A novel amphibian hypothalamic neuropeptide : Isolation, localization, and biological activity. / Koda, Aya; Ukena, Kazuyoshi; Teranishi, Hitoshi; Ohta, Shinji; Yamamoto, Kazutoshi; Kikuyama, Sakae; Tsutsui, Kazuyoshi.

In: Endocrinology, Vol. 143, No. 2, 2002, p. 411-419.

Research output: Contribution to journalArticle

Koda, A, Ukena, K, Teranishi, H, Ohta, S, Yamamoto, K, Kikuyama, S & Tsutsui, K 2002, 'A novel amphibian hypothalamic neuropeptide: Isolation, localization, and biological activity', Endocrinology, vol. 143, no. 2, pp. 411-419. https://doi.org/10.1210/en.143.2.411
Koda, Aya ; Ukena, Kazuyoshi ; Teranishi, Hitoshi ; Ohta, Shinji ; Yamamoto, Kazutoshi ; Kikuyama, Sakae ; Tsutsui, Kazuyoshi. / A novel amphibian hypothalamic neuropeptide : Isolation, localization, and biological activity. In: Endocrinology. 2002 ; Vol. 143, No. 2. pp. 411-419.
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