A novel approach to Au nanoparticle-based identification of DNA nanoarrays

Guo Jun Zhang*, Takashi Tanii, Yuzo Kanari, Chiaki Yasumuro, Takashi Matsukawa, Meishoku Masahara, Iwao Ohdomari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The combination of electron beam lithography and gold nanoparticle-based detection method is subject to a novel high-resolution approach to detecting DNA nanoarrays. In this work, gold nanoparticle-based detection of DNA hybridization on nanostructured arrays is presented. The nanostructured arrays were created by electron beam lithography of a self-assembled monolayer. Amine groups, which are active moieties and are used for attachment of DNA, were introduced to the nanostructures, and the amine-modified structures were characterized by scanning Maxwell-stress microscopy (SMM) for seeing the modification process. The DNA probe covalently immobilized within the nanostructures was hybridized with a biotinylated target DNA. Streptavidin-gold conjugate was then bound to the biotin, thereby assembling inside the nanostructured arrays. The sequence-specific hybridization was imaged by atomic force microscopy (AFM). On the other hand, the activity of the DNA molecules within the nanostructured arrays was verified by fluorescence microscopy using streptavidin-Cy 5 conjugate instead of streptavidin-gold nanoparticles conjugate. On the basis of fluorescent detection, an alternative method has been developed for detection of DNA nanostructures, which will benefit the development of DNA chips.

Original languageEnglish
Pages (from-to)4773-4778
Number of pages6
JournalFrontiers in Bioscience
Issue number12
Publication statusPublished - 2007 May 1


  • Au nanoparticles
  • DNA
  • Hybridization
  • Nanoarrays
  • Self-assembled monolayer
  • Silicon substrate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


Dive into the research topics of 'A novel approach to Au nanoparticle-based identification of DNA nanoarrays'. Together they form a unique fingerprint.

Cite this