A novel approach to Au nanoparticle-based identification of DNA nanoarrays

Guo Jun Zhang, Takashi Tanii, Yuzo Kanari, Chiaki Yasumuro, Takashi Matsukawa, Meishoku Masahara, Iwao Ohdomari

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The combination of electron beam lithography and gold nanoparticle-based detection method is subject to a novel high-resolution approach to detecting DNA nanoarrays. In this work, gold nanoparticle-based detection of DNA hybridization on nanostructured arrays is presented. The nanostructured arrays were created by electron beam lithography of a self-assembled monolayer. Amine groups, which are active moieties and are used for attachment of DNA, were introduced to the nanostructures, and the amine-modified structures were characterized by scanning Maxwell-stress microscopy (SMM) for seeing the modification process. The DNA probe covalently immobilized within the nanostructures was hybridized with a biotinylated target DNA. Streptavidin-gold conjugate was then bound to the biotin, thereby assembling inside the nanostructured arrays. The sequence-specific hybridization was imaged by atomic force microscopy (AFM). On the other hand, the activity of the DNA molecules within the nanostructured arrays was verified by fluorescence microscopy using streptavidin-Cy 5 conjugate instead of streptavidin-gold nanoparticles conjugate. On the basis of fluorescent detection, an alternative method has been developed for detection of DNA nanostructures, which will benefit the development of DNA chips.

    Original languageEnglish
    Pages (from-to)4773-4778
    Number of pages6
    JournalFrontiers in Bioscience
    Volume12
    Issue number12
    DOIs
    Publication statusPublished - 2007 May 1

    Fingerprint

    Nanoparticles
    Gold
    Streptavidin
    Nanostructures
    DNA
    Electron beam lithography
    Amines
    Electrons
    Atomic Force Microscopy
    DNA Probes
    Biotin
    Oligonucleotide Array Sequence Analysis
    Fluorescence Microscopy
    Fluorescence microscopy
    Self assembled monolayers
    Microscopy
    Atomic force microscopy
    Microscopic examination
    Scanning
    Molecules

    Keywords

    • Au nanoparticles
    • DNA
    • Hybridization
    • Nanoarrays
    • Self-assembled monolayer
    • Silicon substrate

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)
    • Medicine(all)

    Cite this

    Zhang, G. J., Tanii, T., Kanari, Y., Yasumuro, C., Matsukawa, T., Masahara, M., & Ohdomari, I. (2007). A novel approach to Au nanoparticle-based identification of DNA nanoarrays. Frontiers in Bioscience, 12(12), 4773-4778. https://doi.org/10.2741/2425

    A novel approach to Au nanoparticle-based identification of DNA nanoarrays. / Zhang, Guo Jun; Tanii, Takashi; Kanari, Yuzo; Yasumuro, Chiaki; Matsukawa, Takashi; Masahara, Meishoku; Ohdomari, Iwao.

    In: Frontiers in Bioscience, Vol. 12, No. 12, 01.05.2007, p. 4773-4778.

    Research output: Contribution to journalArticle

    Zhang, GJ, Tanii, T, Kanari, Y, Yasumuro, C, Matsukawa, T, Masahara, M & Ohdomari, I 2007, 'A novel approach to Au nanoparticle-based identification of DNA nanoarrays', Frontiers in Bioscience, vol. 12, no. 12, pp. 4773-4778. https://doi.org/10.2741/2425
    Zhang GJ, Tanii T, Kanari Y, Yasumuro C, Matsukawa T, Masahara M et al. A novel approach to Au nanoparticle-based identification of DNA nanoarrays. Frontiers in Bioscience. 2007 May 1;12(12):4773-4778. https://doi.org/10.2741/2425
    Zhang, Guo Jun ; Tanii, Takashi ; Kanari, Yuzo ; Yasumuro, Chiaki ; Matsukawa, Takashi ; Masahara, Meishoku ; Ohdomari, Iwao. / A novel approach to Au nanoparticle-based identification of DNA nanoarrays. In: Frontiers in Bioscience. 2007 ; Vol. 12, No. 12. pp. 4773-4778.
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