A novel avian hypothalamic peptide inhibiting gonadotropin release

Kazuyoshi Tsutsui*, Etsuko Saigoh, Kazuyoshi Ukena, Hitoshi Teranishi, Yuko Fujisawa, Motoshi Kikuchi, Susumu Ishii, Peter J. Sharp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

673 Citations (Scopus)


The neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), which was originally isolated from mammals and subsequently from nonmammals. To date, however, an inhibitory peptide of gonadotropin release is unknown in vertebrates. Here we show, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release. Acetic acid extracts of quail brains were passed through C-18 reversed-phase cartridges, and then the retained material was subjected to the reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). The peptide was isolated from avian brain and shown to have the sequence Ser-IIe-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2. Cell bodies and terminals containing this peptide were localized immunohistochemically in the paraventricular nucleus and median eminence, respectively. This peptide inhibited, in a dose-related way, gonadotropin release from cultured quail anterior pituitaries. This is the first hypothalamic peptide inhibiting gonadotropin release reported in a vertebrate. We therefore term it gonadotropin-inhibitory hormone (GnIH). (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)661-667
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2000 Aug 28
Externally publishedYes


  • Characterization
  • Enzyme-linked immunosorbent assay
  • Gonadotropin
  • Hypothalamic peptide
  • Immunohistochemistry
  • Isolation
  • Pituitary
  • Quail
  • Radioimmunoassay
  • RFamide peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


Dive into the research topics of 'A novel avian hypothalamic peptide inhibiting gonadotropin release'. Together they form a unique fingerprint.

Cite this