A novel avian hypothalamic peptide inhibiting gonadotropin release

Kazuyoshi Tsutsui, Etsuko Saigoh, Kazuyoshi Ukena, Hitoshi Teranishi, Yuko Fujisawa, Motoshi Kikuchi, Susumu Ishii, Peter J. Sharp

Research output: Contribution to journalArticle

571 Citations (Scopus)


The neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), which was originally isolated from mammals and subsequently from nonmammals. To date, however, an inhibitory peptide of gonadotropin release is unknown in vertebrates. Here we show, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release. Acetic acid extracts of quail brains were passed through C-18 reversed-phase cartridges, and then the retained material was subjected to the reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). The peptide was isolated from avian brain and shown to have the sequence Ser-IIe-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2. Cell bodies and terminals containing this peptide were localized immunohistochemically in the paraventricular nucleus and median eminence, respectively. This peptide inhibited, in a dose-related way, gonadotropin release from cultured quail anterior pituitaries. This is the first hypothalamic peptide inhibiting gonadotropin release reported in a vertebrate. We therefore term it gonadotropin-inhibitory hormone (GnIH). (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)661-667
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2000 Aug 28
Externally publishedYes


  • Characterization
  • Enzyme-linked immunosorbent assay
  • Gonadotropin
  • Hypothalamic peptide
  • Immunohistochemistry
  • Isolation
  • Pituitary
  • Quail
  • Radioimmunoassay
  • RFamide peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Tsutsui, K., Saigoh, E., Ukena, K., Teranishi, H., Fujisawa, Y., Kikuchi, M., Ishii, S., & Sharp, P. J. (2000). A novel avian hypothalamic peptide inhibiting gonadotropin release. Biochemical and Biophysical Research Communications, 275(2), 661-667. https://doi.org/10.1006/bbrc.2000.3350