A novel carotenoid biosynthetic route via oxidosqualene

Yusuke Otani; Takashi Maoka; Shigeko Kawai-Noma; Kyoichi Saito; Daisuke Umeno

doi:10.1016/j.bbrc.2022.01.105

A novel carotenoid biosynthetic route via oxidosqualene

Yusuke Otani, Takashi Maoka, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

Original language	English
Pages (from-to)	75-80
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	599
DOIs	https://doi.org/10.1016/j.bbrc.2022.01.105
Publication status	Published - 2022 Apr 9

Keywords

Carotenoid
Non-natural routes
Oxidosqualene
Squalene
Triterpenoid

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2022.01.105

Cite this

@article{7ab98e4113644be2a4e4506075d4e058,

title = "A novel carotenoid biosynthetic route via oxidosqualene",

abstract = "Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.",

keywords = "Carotenoid, Non-natural routes, Oxidosqualene, Squalene, Triterpenoid",

author = "Yusuke Otani and Takashi Maoka and Shigeko Kawai-Noma and Kyoichi Saito and Daisuke Umeno",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS Kakenhi-Grants 16H06450 , 18H01791 , 21H01721 ] and by JSPS KAKENHI Grant Number 20J11505 Foundation to Y.O. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

year = "2022",

month = apr,

day = "9",

doi = "10.1016/j.bbrc.2022.01.105",

language = "English",

volume = "599",

pages = "75--80",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - A novel carotenoid biosynthetic route via oxidosqualene

AU - Otani, Yusuke

AU - Maoka, Takashi

AU - Kawai-Noma, Shigeko

AU - Saito, Kyoichi

AU - Umeno, Daisuke

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS Kakenhi-Grants 16H06450 , 18H01791 , 21H01721 ] and by JSPS KAKENHI Grant Number 20J11505 Foundation to Y.O. Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/4/9

Y1 - 2022/4/9

N2 - Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

AB - Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

KW - Carotenoid

KW - Non-natural routes

KW - Oxidosqualene

KW - Squalene

KW - Triterpenoid

UR - http://www.scopus.com/inward/record.url?scp=85124516261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85124516261&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2022.01.105

DO - 10.1016/j.bbrc.2022.01.105

M3 - Article

C2 - 35176628

AN - SCOPUS:85124516261

SN - 0006-291X

VL - 599

SP - 75

EP - 80

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

A novel carotenoid biosynthetic route via oxidosqualene

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this