A novel function for nucleoside diphosphate kinase in drosophila

Hiroko Inoue, Masami Takahashi, Akira Oomori, Mariko Sekiguchi, Tohru Yoshioka

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Nucleoside diphosphate (NDP) kinase is an enzyme that transfers the γ-phosphate of nucleoside triphosphates to nucleoside diphosphates. Besides this well-defined role, recent evidence suggests that NDP kinase may be implicated in a wide variety of essential cellular processes. In this paper, we showed that the NDP kinase of Drosophila exhibited protein kinase activity as well as autophosporylation. Ovalbumin was phosphorylated with guanosine 5'-(3-O-thio)triphosphate (GTPγS) or ATP. Protein kinase activity was not detected in NDP kinase mutant, abnormal wing discs (awd). These results suggest that this activity could be one of the functions of NDP kinase essential for normal fly development, since awd gene is lethal.

    Original languageEnglish
    Pages (from-to)887-892
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume218
    Issue number3
    DOIs
    Publication statusPublished - 1996 Jan 26

    Fingerprint

    Nucleoside-Diphosphate Kinase
    Drosophila
    Nucleosides
    Protein Kinases
    Lethal Genes
    Guanosine 5'-O-(3-Thiotriphosphate)
    Drosophila Proteins
    Diphosphates
    Ovalbumin
    Diptera
    Genes
    Adenosine Triphosphate
    Phosphates
    Enzymes

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    A novel function for nucleoside diphosphate kinase in drosophila. / Inoue, Hiroko; Takahashi, Masami; Oomori, Akira; Sekiguchi, Mariko; Yoshioka, Tohru.

    In: Biochemical and Biophysical Research Communications, Vol. 218, No. 3, 26.01.1996, p. 887-892.

    Research output: Contribution to journalArticle

    Inoue, H, Takahashi, M, Oomori, A, Sekiguchi, M & Yoshioka, T 1996, 'A novel function for nucleoside diphosphate kinase in drosophila', Biochemical and Biophysical Research Communications, vol. 218, no. 3, pp. 887-892. https://doi.org/10.1006/bbrc.1996.0158
    Inoue, Hiroko ; Takahashi, Masami ; Oomori, Akira ; Sekiguchi, Mariko ; Yoshioka, Tohru. / A novel function for nucleoside diphosphate kinase in drosophila. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 218, No. 3. pp. 887-892.
    @article{33cae82f804c4870a27a12d110fa67e1,
    title = "A novel function for nucleoside diphosphate kinase in drosophila",
    abstract = "Nucleoside diphosphate (NDP) kinase is an enzyme that transfers the γ-phosphate of nucleoside triphosphates to nucleoside diphosphates. Besides this well-defined role, recent evidence suggests that NDP kinase may be implicated in a wide variety of essential cellular processes. In this paper, we showed that the NDP kinase of Drosophila exhibited protein kinase activity as well as autophosporylation. Ovalbumin was phosphorylated with guanosine 5'-(3-O-thio)triphosphate (GTPγS) or ATP. Protein kinase activity was not detected in NDP kinase mutant, abnormal wing discs (awd). These results suggest that this activity could be one of the functions of NDP kinase essential for normal fly development, since awd gene is lethal.",
    author = "Hiroko Inoue and Masami Takahashi and Akira Oomori and Mariko Sekiguchi and Tohru Yoshioka",
    year = "1996",
    month = "1",
    day = "26",
    doi = "10.1006/bbrc.1996.0158",
    language = "English",
    volume = "218",
    pages = "887--892",
    journal = "Biochemical and Biophysical Research Communications",
    issn = "0006-291X",
    publisher = "Academic Press Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - A novel function for nucleoside diphosphate kinase in drosophila

    AU - Inoue, Hiroko

    AU - Takahashi, Masami

    AU - Oomori, Akira

    AU - Sekiguchi, Mariko

    AU - Yoshioka, Tohru

    PY - 1996/1/26

    Y1 - 1996/1/26

    N2 - Nucleoside diphosphate (NDP) kinase is an enzyme that transfers the γ-phosphate of nucleoside triphosphates to nucleoside diphosphates. Besides this well-defined role, recent evidence suggests that NDP kinase may be implicated in a wide variety of essential cellular processes. In this paper, we showed that the NDP kinase of Drosophila exhibited protein kinase activity as well as autophosporylation. Ovalbumin was phosphorylated with guanosine 5'-(3-O-thio)triphosphate (GTPγS) or ATP. Protein kinase activity was not detected in NDP kinase mutant, abnormal wing discs (awd). These results suggest that this activity could be one of the functions of NDP kinase essential for normal fly development, since awd gene is lethal.

    AB - Nucleoside diphosphate (NDP) kinase is an enzyme that transfers the γ-phosphate of nucleoside triphosphates to nucleoside diphosphates. Besides this well-defined role, recent evidence suggests that NDP kinase may be implicated in a wide variety of essential cellular processes. In this paper, we showed that the NDP kinase of Drosophila exhibited protein kinase activity as well as autophosporylation. Ovalbumin was phosphorylated with guanosine 5'-(3-O-thio)triphosphate (GTPγS) or ATP. Protein kinase activity was not detected in NDP kinase mutant, abnormal wing discs (awd). These results suggest that this activity could be one of the functions of NDP kinase essential for normal fly development, since awd gene is lethal.

    UR - http://www.scopus.com/inward/record.url?scp=0030033064&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030033064&partnerID=8YFLogxK

    U2 - 10.1006/bbrc.1996.0158

    DO - 10.1006/bbrc.1996.0158

    M3 - Article

    VL - 218

    SP - 887

    EP - 892

    JO - Biochemical and Biophysical Research Communications

    JF - Biochemical and Biophysical Research Communications

    SN - 0006-291X

    IS - 3

    ER -