A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2

Takushi Togashi, Dong Kug Choi, Todd D. Taylor, Yutaka Suzuki, Sumio Sugano, Masahira Hattori, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Based on a detailed sequence of the distal Down syndrome critical region (DSCR), we predicted and molecularly cloned a novel gene, designated DSCR5. We determined the sequences of expressed sequence tags (ESTs) that almost matched the predicted cDNA sequence of DSCR5. Northern blot analysis showed that DSCR5 is expressed in several tissues including the liver, skeletal muscle, heart, pancreas and testis. To determine the 5′-end of DSCR5, the oligo-capping method was employed. Combining the EST sequence data and that from the oligo-capping experiments, we obtained the full-length cDNA sequence of DSCR5. DSCR5 had at least four types of alternatively spliced variants. According to the number of exons, they could be classified into two subtypes: DSCR5α and DSCR5β. DSCR5α includes three splice variant subtypes, DSCR5α1, α2 and α3, which each has different first non-coding exon. In addition, the most abundantly isolated form, DSCR5α1, shows microheterogeneity of the mRNA start site. Comparison of the sequences between the predicted cDNA and the molecularly cloned cDNA revealed that the computer programs had limited validity to correctly predict the terminal exons. Thus, molecular cloning should always be required to complement the inadequacy of the computer predictions.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalDNA Research
Volume7
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Complementary DNA
Chromosomes
Exons
Expressed Sequence Tags
Genes
Molecular Cloning
Northern Blotting
Testis
Pancreas
Skeletal Muscle
Software
Messenger RNA
Down Syndrome Critical Region
Liver

Keywords

  • cDNA cloning
  • Down syndrome
  • Oligo-capping

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Togashi, T., Choi, D. K., Taylor, T. D., Suzuki, Y., Sugano, S., Hattori, M., & Sakaki, Y. (2000). A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2. DNA Research, 7(3), 207-212.

A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2. / Togashi, Takushi; Choi, Dong Kug; Taylor, Todd D.; Suzuki, Yutaka; Sugano, Sumio; Hattori, Masahira; Sakaki, Yoshiyuki.

In: DNA Research, Vol. 7, No. 3, 2000, p. 207-212.

Research output: Contribution to journalArticle

Togashi, T, Choi, DK, Taylor, TD, Suzuki, Y, Sugano, S, Hattori, M & Sakaki, Y 2000, 'A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2', DNA Research, vol. 7, no. 3, pp. 207-212.
Togashi T, Choi DK, Taylor TD, Suzuki Y, Sugano S, Hattori M et al. A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2. DNA Research. 2000;7(3):207-212.
Togashi, Takushi ; Choi, Dong Kug ; Taylor, Todd D. ; Suzuki, Yutaka ; Sugano, Sumio ; Hattori, Masahira ; Sakaki, Yoshiyuki. / A novel gene, DSCR5, from the distal Down syndrome critical region on chromosome 21q22.2. In: DNA Research. 2000 ; Vol. 7, No. 3. pp. 207-212.
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