A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae

Satoshi Yoshida, Yoshikazu Ohya, Mark Goebl, Akihiko Nakano, Yasuhiro Anraku

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Abstract

A staurosporine-sensitive mutation (stt1) in yeast has been found in the PKC1 gene that encodes a protein kinase C homologue (Yoshida, S., Ikeda, E., Uno, I., and Mitsuzawa, H. (1992) Mol. Gen. Genet. 231, 337-344). We report here another staurosporine-sensitive mutant, stt4, which shows very similar phenotypes to that of the stt1 mutant. The stt4 temperature-sensitive mutant arrests mostly in G2/M phase at 37 °C, and the stt4 deletion mutant shows an osmoremedial phenotype. Staurosporine sensitivity of the stt4 mutant was suppressed by overexpression of PKC1/STT1, indicating genetic interaction between stt4 and pkc1/stt1. The nucleotide sequence of STT4 predicts a hydrophilic protein composed of 1,900 amino acid residues, with 26% sequence identity to the yeast VPS34 gene product and 27% to the catalytic subunit of mammalian phosphatidylinositol (PI) 3-kinase, respectively. Cell homogenates of the stt4 deletion mutant show normal PI3-kinase activity but lack most of the PI4-kinase activity that is detected in the wild-type. We conclude that STT4 encodes a yeast PI4-kinase that functions in the PKC1 protein kinase pathway.

Original languageEnglish
Pages (from-to)1166-1171
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number2
Publication statusPublished - 1994 Jan 14
Externally publishedYes

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1-Phosphatidylinositol 4-Kinase
Staurosporine
Yeast
Protein Kinases
Saccharomyces cerevisiae
Genes
Yeasts
Phosphotransferases
Phosphatidylinositol 3-Kinase
Viverridae
Phenotype
G2 Phase
Phosphatidylinositol 3-Kinases
Cell Division
Protein Kinase C
Catalytic Domain
Nucleotides
Amino Acids
Mutation
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae. / Yoshida, Satoshi; Ohya, Yoshikazu; Goebl, Mark; Nakano, Akihiko; Anraku, Yasuhiro.

In: Journal of Biological Chemistry, Vol. 269, No. 2, 14.01.1994, p. 1166-1171.

Research output: Contribution to journalArticle

Yoshida, Satoshi ; Ohya, Yoshikazu ; Goebl, Mark ; Nakano, Akihiko ; Anraku, Yasuhiro. / A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 2. pp. 1166-1171.
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