A novel L-amino acid ligase from bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis

Kuniki Kino, Toshinobu Arai, Daisuke Tateiwa

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    L-Amino acid ligase catalyzes dipeptide synthesis from unprotected L-amino acids in an ATP-dependent manner. We have purified a new L-amino acid ligase, RizA, which synthesizes dipeptides whose N-terminus is Arg, from Bacillus subtilis NBRC3134, a microorganism that produces a rhizocticin peptide antibiotic. It was suggested that RizA is probably involved in rhizocticin biosynthesis. In this study, we performed sequence analysis of unknown regions around rizA, and newly identified a gene that encodes a protein that possesses an ATP-grasp motif upstream of rizA. This gene was designated rizB, and its recombinant protein was prepared. Recombinant RizB synthesized homo-oligo-mers of branched-chain L-amino acids and L-methionine consisting of two to five amino acids in an ATP-dependent manner. RizB also synthesized various heteropeptides. Further examination showed that RizB might elongate a peptide chain at the N-terminus. This is the first report on an L-amino acid ligase catalyzing oligopeptide synthesis.

    Original languageEnglish
    Pages (from-to)129-134
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume74
    Issue number1
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Oligopeptides
    Bacilli
    Ligases
    Bacillus subtilis
    Amino Acids
    Dipeptides
    Adenosine Triphosphate
    Genes
    Branched Chain Amino Acids
    Peptides
    Hand Strength
    Recombinant Proteins
    Methionine
    Biosynthesis
    Sequence Analysis
    Microorganisms
    Anti-Bacterial Agents
    Proteins

    Keywords

    • L-amino acid ligase
    • Oligopeptide synthesis
    • Rhizocticin

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Analytical Chemistry
    • Organic Chemistry

    Cite this

    A novel L-amino acid ligase from bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis. / Kino, Kuniki; Arai, Toshinobu; Tateiwa, Daisuke.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 1, 2010, p. 129-134.

    Research output: Contribution to journalArticle

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