A novel nitric oxide synthase expressed specifically in the olfactory center

Ryota Matsuo, Etsuro Ito

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nitric oxide (NO) plays important roles in the olfactory center of various animals. In the terrestrial slug, NO is indispensable for field potential oscillation in the higher olfactory center, the procerebrum (PC), and also for odor learning. Here we identify a novel NO synthase (NOS) gene, limNOS2, in the terrestrial slug. The mRNA (∼10 kb) of limNOS2 encodes a protein consisting of 1616 amino acids, including a PDZ domain. The protein has 70.0% sequence identity with the previously identified limNOS1 gene. In contrast to limNOS1, however, limNOS2 is expressed specifically in the PC. Moreover, most of the cells in the PC contain limNOS2 mRNA, indicating that the nonbursting neurons, the major constituent of the PC, have this mRNA. The expression pattern of limNOS2 conforms well to the pattern of NOS enzymatic histochemical staining. Our present findings indicate that limNOS2 is responsible for most of the NO generation in the PC.

Original languageEnglish
Pages (from-to)724-728
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number4
DOIs
Publication statusPublished - 2009 Sep 4
Externally publishedYes

Fingerprint

Nitric Oxide Synthase
Nitric Oxide
Gastropoda
Messenger RNA
Genes
PDZ Domains
Odors
Neurons
Animals
Proteins
Learning
Staining and Labeling
Amino Acids
Odorants

Keywords

  • Limax
  • Local field potential oscillation
  • NADPH-diaphorase staining
  • Nitric oxide synthase
  • Nonbursting neurons
  • Olfactory center
  • PDZ domain
  • Procerebrum
  • Terrestrial slug

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

A novel nitric oxide synthase expressed specifically in the olfactory center. / Matsuo, Ryota; Ito, Etsuro.

In: Biochemical and Biophysical Research Communications, Vol. 386, No. 4, 04.09.2009, p. 724-728.

Research output: Contribution to journalArticle

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