A nucleoside kinase as a dual selector for genetic switches and circuits

Yohei Tashiro; Hiroki Fukutomi; Kei Terakubo; Kyoichi Saito; Daisuke Umeno

doi:10.1093/nar/gkq1070

A nucleoside kinase as a dual selector for genetic switches and circuits

Yohei Tashiro, Hiroki Fukutomi, Kei Terakubo, Kyoichi Saito, Daisuke Umeno^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.

Original language	English
Pages (from-to)	e12
Journal	Nucleic acids research
Volume	39
Issue number	3
DOIs	https://doi.org/10.1093/nar/gkq1070
Publication status	Published - 2011 Feb
Externally published	Yes

ASJC Scopus subject areas

Genetics

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abstract = "The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.",

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AU - Saito, Kyoichi

AU - Umeno, Daisuke

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A nucleoside kinase as a dual selector for genetic switches and circuits

Abstract

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