A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats

Zsolt Radak, Akihiro Nakamura, Hideko Nakamoto, Katsumi Asano, Hideki Ohno, Sataro Goto

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.

Original languageEnglish
Pages (from-to)439-441
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume435
Issue number3
DOIs
Publication statusPublished - 1998 Feb 18
Externally publishedYes

Fingerprint

Rats
Derivatives
Lung
Glutamate-Ammonia Ligase
Running
Proteins
Exercise
Free Radicals
Molecular Weight
Western Blotting
Molecular weight
Recovery
Oxidation

Keywords

  • Exercise
  • Glutamine synthetase
  • Lung
  • Protein oxidation

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats. / Radak, Zsolt; Nakamura, Akihiro; Nakamoto, Hideko; Asano, Katsumi; Ohno, Hideki; Goto, Sataro.

In: Pflugers Archiv European Journal of Physiology, Vol. 435, No. 3, 18.02.1998, p. 439-441.

Research output: Contribution to journalArticle

Radak, Zsolt ; Nakamura, Akihiro ; Nakamoto, Hideko ; Asano, Katsumi ; Ohno, Hideki ; Goto, Sataro. / A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats. In: Pflugers Archiv European Journal of Physiology. 1998 ; Vol. 435, No. 3. pp. 439-441.
@article{6743dfa60f3844bab3953ef1c861bde9,
title = "A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats",
abstract = "It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30{\%} in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.",
keywords = "Exercise, Glutamine synthetase, Lung, Protein oxidation",
author = "Zsolt Radak and Akihiro Nakamura and Hideko Nakamoto and Katsumi Asano and Hideki Ohno and Sataro Goto",
year = "1998",
month = "2",
day = "18",
doi = "10.1007/s004240050537",
language = "English",
volume = "435",
pages = "439--441",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats

AU - Radak, Zsolt

AU - Nakamura, Akihiro

AU - Nakamoto, Hideko

AU - Asano, Katsumi

AU - Ohno, Hideki

AU - Goto, Sataro

PY - 1998/2/18

Y1 - 1998/2/18

N2 - It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.

AB - It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.

KW - Exercise

KW - Glutamine synthetase

KW - Lung

KW - Protein oxidation

UR - http://www.scopus.com/inward/record.url?scp=0031909094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031909094&partnerID=8YFLogxK

U2 - 10.1007/s004240050537

DO - 10.1007/s004240050537

M3 - Article

C2 - 9426304

AN - SCOPUS:0031909094

VL - 435

SP - 439

EP - 441

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 3

ER -