A period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats

Zsolt Radák, Akihiro Nakamura, Hideko Nakamoto, Katsumi Asano, Hideki Ohno, Sataro Goto

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.

Original languageEnglish
Pages (from-to)439-441
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume435
Issue number3
DOIs
Publication statusPublished - 1998 Feb 18
Externally publishedYes

Keywords

  • Exercise
  • Glutamine synthetase
  • Lung
  • Protein oxidation

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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