A possible role of the C-terminal domain of the RecA protein: A gateway model for double-stranded DNA binding

Hitoshi Kurumizaka, Hideki Aihara, Shukuko Ikawa, Takamitsu Kashima, L. Rochelle Bazemore, Katsumi Kawasaki, Akinori Sarai, Charles M. Radding, Takehiko Shibata

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

According to the crystal structure, the RecA protein has a domain near the C terminus consisting of amino acid residues 270-328 (from the N terminus). Our model building pointed out the possibility that this domain is a part of 'gateway' through which double-stranded DNA finds a path for direct contact with single-stranded DNA within a presynaptic RecA filament in the search for homology. To test this possible function of the domain, we made mutant RecA proteins by site-directed single (or double, in one case) replacement of 2 conserved basic amino acid residues and 5 among 9 nonconserved basic amino acid residues in the domain. Replacement of either of the 2 conserved amino acid residues caused deficiencies in repair of UV- damaged DNA, an in vivo function of RecA protein, whereas the replacement of most (except one) of the tested nonconserved ones gave little or no effect. Purified mutant RecA proteins showed no (or only slight) deficiencies in the formation of presynaptic filaments as assessed by various assays. However, presynaptic filaments of both proteins that had replacement of a conserved amino acid residue had significant defects in binding to and pairing with duplex DNA (secondary binding). These results are consistent with our model that the conserved amino acid residues in the C-terminal domain have a direct role in double-stranded DNA binding and that they constitute a part of a gateway for homologous recognition.

Original languageEnglish
Pages (from-to)33515-33524
Number of pages10
JournalJournal of Biological Chemistry
Volume271
Issue number52
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Rec A Recombinases
Staphylococcal Protein A
Amino Acids
Basic Amino Acids
DNA
Mutant Proteins
Single-Stranded DNA
Assays
Repair
Crystal structure
Defects
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kurumizaka, H., Aihara, H., Ikawa, S., Kashima, T., Rochelle Bazemore, L., Kawasaki, K., ... Shibata, T. (1996). A possible role of the C-terminal domain of the RecA protein: A gateway model for double-stranded DNA binding. Journal of Biological Chemistry, 271(52), 33515-33524. https://doi.org/10.1074/jbc.271.52.33515

A possible role of the C-terminal domain of the RecA protein : A gateway model for double-stranded DNA binding. / Kurumizaka, Hitoshi; Aihara, Hideki; Ikawa, Shukuko; Kashima, Takamitsu; Rochelle Bazemore, L.; Kawasaki, Katsumi; Sarai, Akinori; Radding, Charles M.; Shibata, Takehiko.

In: Journal of Biological Chemistry, Vol. 271, No. 52, 1996, p. 33515-33524.

Research output: Contribution to journalArticle

Kurumizaka, H, Aihara, H, Ikawa, S, Kashima, T, Rochelle Bazemore, L, Kawasaki, K, Sarai, A, Radding, CM & Shibata, T 1996, 'A possible role of the C-terminal domain of the RecA protein: A gateway model for double-stranded DNA binding', Journal of Biological Chemistry, vol. 271, no. 52, pp. 33515-33524. https://doi.org/10.1074/jbc.271.52.33515
Kurumizaka, Hitoshi ; Aihara, Hideki ; Ikawa, Shukuko ; Kashima, Takamitsu ; Rochelle Bazemore, L. ; Kawasaki, Katsumi ; Sarai, Akinori ; Radding, Charles M. ; Shibata, Takehiko. / A possible role of the C-terminal domain of the RecA protein : A gateway model for double-stranded DNA binding. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 52. pp. 33515-33524.
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