A prodigiosin analogue inactivates NADPH oxidase in macrophage cells by inhibiting assembly of p47phox and Rac

Takuji Nakashima*, Takashi Iwashita, Tsuyoshi Fujita, Emiko Sato, Yoshimi Niwano, Masahiro Kohno, Shunsuke Kuwahara, Nobuyuki Harada, Satoshi Takeshita, Tatsuya Oda

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Prodigiosins are natural red pigments that have multi-biological activities. Recently, we discovered a marine bacterial strain, which produces a red pigment. Extensive two-dimensional nuclear magnetic resonance and mass spectrometry analysis showed that the pigment is a prodigiosin analogue (PG-L-1). Here, we investigated the effect of PG-L-1 on NADPH oxidase activity in macrophage cells. PG-L-1 significantly inhibited superoxide anion (O 2-) production by phorbol 12-myristate 13-acetate (PMA)-stimulated RAW 264.7 cells, a mouse macrophage cell line. The ED 50 value was estimated to be ∼0.3 μM. PG-L-1 had no direct scavenging effect on O2- generated by hypoxanthine/ xanthine oxidase system in electron spin resonance-spin trapping determinations, suggesting that this compound directly acts on the O2- production system, NADPH oxidase, in macrophage cells. We further investigated the effect of PG-L-1 on the behaviour of the cytosolic components of the NADPH oxidase, p67phox, p47phox, p40phox, Rac and protein kinase C (PKC), in PMA-stimulated RAW 264.7 cells. Although PG-L-1 showed no effect on the activation of PKC, the immunoblotting analysis using specific antibodies showed that PG-L-1 strongly inhibits the association of p47phox and Rac in the plasma membrane of PMA-stimulated RAW 264.7 cells. These results suggest that PG-L-1 inactivates NADPH oxidase through the inhibition of the binding of p47phox and Rac to the membrane components of NADPH oxidase.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalJournal of biochemistry
Volume143
Issue number1
DOIs
Publication statusPublished - 2008 Jan
Externally publishedYes

Keywords

  • NADPH oxidase inhibitor
  • Prodigiosin
  • Rac protein
  • Superoxide
  • p47phox

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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