TY - JOUR
T1 - A rat model of saliva secretory immunoglobulin
T2 - A suppression caused by intense exercise
AU - Kimura, F.
AU - Aizawa, K.
AU - Tanabe, K.
AU - Shimizu, K.
AU - Kon, M.
AU - Lee, H.
AU - Akimoto, T.
AU - Akama, T.
AU - Kono, I.
PY - 2008/6
Y1 - 2008/6
N2 - We aimed to develop a valid model of immunosuppression induced by intense exercise in rats. Rats were divided into three groups. In the rest (Rest) group, saliva was collected from resting rats on 4 consecutive days. In the exercise (Ex) group, rats ran on a treadmill untill exhaustion (exercise time: 60.0 ± 3.7 min), and their saliva was collected before and after exercise; the salivary glands were removed after exercise. In the control (Con) group, saliva collection and gland removal were also performed, but the rats did not exercise. Secretory immunoglobulin A (SIgA) concentrations in saliva and polymeric immunoglobulin receptor (pIgR) mRNA expression in the glands were measured. There was no significant change in SIgA concentration in the Rest group over 4 days. In the Ex group, SIgA concentration decreased significantly after exercise compared with before, whereas there was no significant change in the Con group. The expression of pIgR mRNA was significantly lower in the Ex group post-exercise than in the Con group. Our procedure for saliva collection appeared suitable, and the exercise-induced SIgA suppression was probably caused by a decline in pIgR mRNA expression. We propose to use this reproducible and reliable rat model of exercise-induced SIgA suppression in future studies.
AB - We aimed to develop a valid model of immunosuppression induced by intense exercise in rats. Rats were divided into three groups. In the rest (Rest) group, saliva was collected from resting rats on 4 consecutive days. In the exercise (Ex) group, rats ran on a treadmill untill exhaustion (exercise time: 60.0 ± 3.7 min), and their saliva was collected before and after exercise; the salivary glands were removed after exercise. In the control (Con) group, saliva collection and gland removal were also performed, but the rats did not exercise. Secretory immunoglobulin A (SIgA) concentrations in saliva and polymeric immunoglobulin receptor (pIgR) mRNA expression in the glands were measured. There was no significant change in SIgA concentration in the Rest group over 4 days. In the Ex group, SIgA concentration decreased significantly after exercise compared with before, whereas there was no significant change in the Con group. The expression of pIgR mRNA was significantly lower in the Ex group post-exercise than in the Con group. Our procedure for saliva collection appeared suitable, and the exercise-induced SIgA suppression was probably caused by a decline in pIgR mRNA expression. We propose to use this reproducible and reliable rat model of exercise-induced SIgA suppression in future studies.
KW - Exhaustive exercise
KW - Rat model
KW - SIgA
KW - URTI
KW - pIgR
UR - http://www.scopus.com/inward/record.url?scp=44349084360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44349084360&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0838.2007.00642.x
DO - 10.1111/j.1600-0838.2007.00642.x
M3 - Article
C2 - 17555544
AN - SCOPUS:44349084360
SN - 0905-7188
VL - 18
SP - 367
EP - 372
JO - Scandinavian Journal of Medicine and Science in Sports
JF - Scandinavian Journal of Medicine and Science in Sports
IS - 3
ER -