A rat model of saliva secretory immunoglobulin: A suppression caused by intense exercise

F. Kimura, K. Aizawa, K. Tanabe, K. Shimizu, M. Kon, H. Lee, Takayuki Akimoto, Takao Akama, I. Kono

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We aimed to develop a valid model of immunosuppression induced by intense exercise in rats. Rats were divided into three groups. In the rest (Rest) group, saliva was collected from resting rats on 4 consecutive days. In the exercise (Ex) group, rats ran on a treadmill untill exhaustion (exercise time: 60.0 ± 3.7 min), and their saliva was collected before and after exercise; the salivary glands were removed after exercise. In the control (Con) group, saliva collection and gland removal were also performed, but the rats did not exercise. Secretory immunoglobulin A (SIgA) concentrations in saliva and polymeric immunoglobulin receptor (pIgR) mRNA expression in the glands were measured. There was no significant change in SIgA concentration in the Rest group over 4 days. In the Ex group, SIgA concentration decreased significantly after exercise compared with before, whereas there was no significant change in the Con group. The expression of pIgR mRNA was significantly lower in the Ex group post-exercise than in the Con group. Our procedure for saliva collection appeared suitable, and the exercise-induced SIgA suppression was probably caused by a decline in pIgR mRNA expression. We propose to use this reproducible and reliable rat model of exercise-induced SIgA suppression in future studies.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalScandinavian Journal of Medicine and Science in Sports
Volume18
Issue number3
DOIs
Publication statusPublished - 2008 Jun

Fingerprint

Secretory Immunoglobulin A
Saliva
Polymeric Immunoglobulin Receptors
Control Groups
Messenger RNA
Salivary Glands
Immunosuppression

Keywords

  • Exhaustive exercise
  • pIgR
  • Rat model
  • SIgA
  • URTI

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

A rat model of saliva secretory immunoglobulin : A suppression caused by intense exercise. / Kimura, F.; Aizawa, K.; Tanabe, K.; Shimizu, K.; Kon, M.; Lee, H.; Akimoto, Takayuki; Akama, Takao; Kono, I.

In: Scandinavian Journal of Medicine and Science in Sports, Vol. 18, No. 3, 06.2008, p. 367-372.

Research output: Contribution to journalArticle

Kimura, F. ; Aizawa, K. ; Tanabe, K. ; Shimizu, K. ; Kon, M. ; Lee, H. ; Akimoto, Takayuki ; Akama, Takao ; Kono, I. / A rat model of saliva secretory immunoglobulin : A suppression caused by intense exercise. In: Scandinavian Journal of Medicine and Science in Sports. 2008 ; Vol. 18, No. 3. pp. 367-372.
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