A simple means to immobilize enzyme into conducting polymers via entrapment

Jun Chen, Bjorn Winther Jensen, Carol Lynam, Orawan Ngamna, Simon Moulton, Weimin Zhang, Gordon G. Wallace

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

Original languageEnglish
JournalElectrochemical and Solid-State Letters
Volume9
Issue number7
DOIs
Publication statusPublished - 2006 Jul
Externally publishedYes

Fingerprint

entrapment
Conducting polymers
conducting polymers
Horseradish Peroxidase
enzymes
Enzymes
washing
Thiophene
thiophenes
immobilization
bioinstrumentation
Biosensors
Washing
phosphates
Phosphates
Ethanol
ethyl alcohol
polymerization
Vapors
Polymerization

ASJC Scopus subject areas

  • Electrochemistry
  • Materials Science(all)

Cite this

Chen, J., Winther Jensen, B., Lynam, C., Ngamna, O., Moulton, S., Zhang, W., & Wallace, G. G. (2006). A simple means to immobilize enzyme into conducting polymers via entrapment. Electrochemical and Solid-State Letters, 9(7). https://doi.org/10.1149/1.2201306

A simple means to immobilize enzyme into conducting polymers via entrapment. / Chen, Jun; Winther Jensen, Bjorn; Lynam, Carol; Ngamna, Orawan; Moulton, Simon; Zhang, Weimin; Wallace, Gordon G.

In: Electrochemical and Solid-State Letters, Vol. 9, No. 7, 07.2006.

Research output: Contribution to journalArticle

Chen, J, Winther Jensen, B, Lynam, C, Ngamna, O, Moulton, S, Zhang, W & Wallace, GG 2006, 'A simple means to immobilize enzyme into conducting polymers via entrapment', Electrochemical and Solid-State Letters, vol. 9, no. 7. https://doi.org/10.1149/1.2201306
Chen, Jun ; Winther Jensen, Bjorn ; Lynam, Carol ; Ngamna, Orawan ; Moulton, Simon ; Zhang, Weimin ; Wallace, Gordon G. / A simple means to immobilize enzyme into conducting polymers via entrapment. In: Electrochemical and Solid-State Letters. 2006 ; Vol. 9, No. 7.
@article{87ffdd87db0944749b8bc76ad49d5dc4,
title = "A simple means to immobilize enzyme into conducting polymers via entrapment",
abstract = "Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5{\%} of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.",
author = "Jun Chen and {Winther Jensen}, Bjorn and Carol Lynam and Orawan Ngamna and Simon Moulton and Weimin Zhang and Wallace, {Gordon G.}",
year = "2006",
month = "7",
doi = "10.1149/1.2201306",
language = "English",
volume = "9",
journal = "Electrochemical and Solid-State Letters",
issn = "1099-0062",
publisher = "Electrochemical Society, Inc.",
number = "7",

}

TY - JOUR

T1 - A simple means to immobilize enzyme into conducting polymers via entrapment

AU - Chen, Jun

AU - Winther Jensen, Bjorn

AU - Lynam, Carol

AU - Ngamna, Orawan

AU - Moulton, Simon

AU - Zhang, Weimin

AU - Wallace, Gordon G.

PY - 2006/7

Y1 - 2006/7

N2 - Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

AB - Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

UR - http://www.scopus.com/inward/record.url?scp=33646865540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646865540&partnerID=8YFLogxK

U2 - 10.1149/1.2201306

DO - 10.1149/1.2201306

M3 - Article

AN - SCOPUS:33646865540

VL - 9

JO - Electrochemical and Solid-State Letters

JF - Electrochemical and Solid-State Letters

SN - 1099-0062

IS - 7

ER -