A simple means to immobilize enzyme into conducting polymers via entrapment

Jun Chen, Bjorn Winther Jensen, Carol Lynam, Orawan Ngamna, Simon Moulton, Weimin Zhang, Gordon G. Wallace

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

Original languageEnglish
JournalElectrochemical and Solid-State Letters
Volume9
Issue number7
DOIs
Publication statusPublished - 2006 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Electrochemistry
  • Materials Science(all)

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    Chen, J., Winther Jensen, B., Lynam, C., Ngamna, O., Moulton, S., Zhang, W., & Wallace, G. G. (2006). A simple means to immobilize enzyme into conducting polymers via entrapment. Electrochemical and Solid-State Letters, 9(7). https://doi.org/10.1149/1.2201306