A simple method for introducing marker-free deletions in the bacillus subtilis genome

Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.

Original languageEnglish
Title of host publicationStrain Engineering
Subtitle of host publicationMethods and Protocols
EditorsJames Williams
Pages345-358
Number of pages14
DOIs
Publication statusPublished - 2011 Sep 23
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume765
ISSN (Print)1064-3745

Keywords

  • Bacillus subtilis
  • Marker-free mutant
  • MazF

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'A simple method for introducing marker-free deletions in the bacillus subtilis genome'. Together they form a unique fingerprint.

Cite this