A simple method for introducing marker-free deletions in the bacillus subtilis genome

Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.

Original languageEnglish
Title of host publicationStrain Engineering
Subtitle of host publicationMethods and Protocols
EditorsJames Williams
Pages345-358
Number of pages14
DOIs
Publication statusPublished - 2011 Sep 23
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume765
ISSN (Print)1064-3745

Keywords

  • Bacillus subtilis
  • Marker-free mutant
  • MazF

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Morimoto, T., Ara, K., Ozaki, K., & Ogasawara, N. (2011). A simple method for introducing marker-free deletions in the bacillus subtilis genome. In J. Williams (Ed.), Strain Engineering: Methods and Protocols (pp. 345-358). (Methods in Molecular Biology; Vol. 765). https://doi.org/10.1007/978-1-61779-197-0_20