Abstract
We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.
| Original language | English |
|---|---|
| Pages (from-to) | 777-781 |
| Number of pages | 5 |
| Journal | DNA and Cell Biology |
| Volume | 9 |
| Issue number | 10 |
| Publication status | Published - 1990 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology