A simplified method for the preparation of transcriptionally active liver nuclear extracts

Masahira Hattori, A. Tugores, L. Veloz, M. Karin, D. A. Brenner

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

Original languageEnglish
Pages (from-to)777-781
Number of pages5
JournalDNA and Cell Biology
Volume9
Issue number10
Publication statusPublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Hattori, M., Tugores, A., Veloz, L., Karin, M., & Brenner, D. A. (1990). A simplified method for the preparation of transcriptionally active liver nuclear extracts. DNA and Cell Biology, 9(10), 777-781.