A single daily meal at the beginning of the active or inactive period inhibits food deprivation-induced fatty liver in mice

Natsumi Aoki, Daisuke Yoshida, Ryosuke Ishikawa, Midori Ando, Kaai Nakamura, Yu Tahara, Shigenobu Shibata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Food deprivation (FD) induces hepatic steatosis in both rodents and humans. Although body composition, age, and sex influence hepatic triglyceride (TG) levels after FD, whether feeding patterns affect FD-induced liver TG increases is unknown. We hypothesized that restricted feeding (RF) of 1 meal per day during the active or inactive period (especially the inactive period) augments FD-induced elevation of liver TGs because RF in the inactive period impairs the circadian rhythm. Triglyceride levels and the expression of genes related to TG metabolism in the liver were examined by a bioassay and real-time reverse transcription-polymerase chain reaction, respectively. In the first experiment, when compared to nonfasted mice, mice that fasted for 24 hours showed a 1.5-fold (FD starting during the inactive period) to 3-fold (FD started during the active period) increase in liver TG levels. This experiment showed that TG levels depend upon the starting time of FD. In the second experiment, mice were given free access to food for 3 hours at the beginning of either the inactive ("supper-only") or the active ("breakfast-only") period for 2 weeks. Restricted feeding inhibited the FD-induced increases in liver and serum TG levels, serum free fatty acids, and the expression of genes related to fatty acid uptake in the liver, including fatty acid transport protein 1 (Fatp1) and 4 (Fatp4). Unexpectedly, compared to free feeding, RF during the active or inactive period resulted in resistance to FD-induced fatty liver. This is the first study to demonstrate that feeding patterns affect FD-induced TG accumulation in the mouse liver.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalNutrition Research
Volume34
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Food Deprivation
Fatty Liver
Meals
Triglycerides
Liver
Feeding Behavior
Fatty Acid Transport Proteins
Gene Expression
Breakfast
Circadian Rhythm
Body Composition
Serum
Nonesterified Fatty Acids
Biological Assay
Reverse Transcription
Rodentia
Fatty Acids

Keywords

  • Circadian rhythm
  • Fatty liver
  • Food deprivation
  • Meal timing
  • Metabolic syndrome
  • Mice

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

A single daily meal at the beginning of the active or inactive period inhibits food deprivation-induced fatty liver in mice. / Aoki, Natsumi; Yoshida, Daisuke; Ishikawa, Ryosuke; Ando, Midori; Nakamura, Kaai; Tahara, Yu; Shibata, Shigenobu.

In: Nutrition Research, Vol. 34, No. 7, 2014, p. 613-622.

Research output: Contribution to journalArticle

Aoki, Natsumi ; Yoshida, Daisuke ; Ishikawa, Ryosuke ; Ando, Midori ; Nakamura, Kaai ; Tahara, Yu ; Shibata, Shigenobu. / A single daily meal at the beginning of the active or inactive period inhibits food deprivation-induced fatty liver in mice. In: Nutrition Research. 2014 ; Vol. 34, No. 7. pp. 613-622.
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AU - Tahara, Yu

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