A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules

Masaru Kato, Yusuke Suwanai, Atsushi Shimojima, Tomofumi Santa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The preparation of nanometer-scale pores, or nanopores, has become easy because of the progress in nanotechnology. Surfactants are promising materials for the preparation of nanostructures containing nanopores, because surfactants form many different phase structures, including cubic, micellar, and lamellar structures. We prepared a gel matrix with a cubic structure from a commercially available surfactant, polyoxyethylene(50) lauryl ether (C12EO50, Adekatol LA-50). This gel matrix had regularly arrayed nanopores between the packed spherical micelles. We used the gel to separate biomolecules by means of slab gel electrophoresis. The gel was applicable to migration of amino acids and peptides; however, larger molecules, such as proteins and single-walled carbon nanotubes, did not migrate through the gel. We concluded that the pore size was too small for the penetration of large molecules, and that only small molecules could penetrate the gel matrix. The migration mechanism of small molecules was similar to that observed in conventional gel electrophoresis. We concluded that the gel matrix prepared from surfactant is a promising matrix for migration and purification of small molecules. We also expect that the gel can be used as a nanoscale filter to trap large molecules, allowing only small molecules to pass.

Original languageEnglish
Pages (from-to)3339-3342
Number of pages4
JournalElectrophoresis
Volume33
Issue number22
DOIs
Publication statusPublished - 2012 Nov
Externally publishedYes

Fingerprint

Nanostructures
Surface-Active Agents
Gels
Molecules
Nanopores
Electrophoresis
Carbon Nanotubes
Nanotechnology
Lamellar structures
Micelles
Biomolecules
Single-walled carbon nanotubes (SWCN)
Phase structure
Ether
Pore size
Purification
Amino Acids
Peptides

Keywords

  • Biomolecule
  • Gel electrophoresis
  • Surfactant

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules. / Kato, Masaru; Suwanai, Yusuke; Shimojima, Atsushi; Santa, Tomofumi.

In: Electrophoresis, Vol. 33, No. 22, 11.2012, p. 3339-3342.

Research output: Contribution to journalArticle

Kato, Masaru ; Suwanai, Yusuke ; Shimojima, Atsushi ; Santa, Tomofumi. / A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules. In: Electrophoresis. 2012 ; Vol. 33, No. 22. pp. 3339-3342.
@article{5d0cf82e06414bf7973dcb035c094458,
title = "A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules",
abstract = "The preparation of nanometer-scale pores, or nanopores, has become easy because of the progress in nanotechnology. Surfactants are promising materials for the preparation of nanostructures containing nanopores, because surfactants form many different phase structures, including cubic, micellar, and lamellar structures. We prepared a gel matrix with a cubic structure from a commercially available surfactant, polyoxyethylene(50) lauryl ether (C12EO50, Adekatol LA-50). This gel matrix had regularly arrayed nanopores between the packed spherical micelles. We used the gel to separate biomolecules by means of slab gel electrophoresis. The gel was applicable to migration of amino acids and peptides; however, larger molecules, such as proteins and single-walled carbon nanotubes, did not migrate through the gel. We concluded that the pore size was too small for the penetration of large molecules, and that only small molecules could penetrate the gel matrix. The migration mechanism of small molecules was similar to that observed in conventional gel electrophoresis. We concluded that the gel matrix prepared from surfactant is a promising matrix for migration and purification of small molecules. We also expect that the gel can be used as a nanoscale filter to trap large molecules, allowing only small molecules to pass.",
keywords = "Biomolecule, Gel electrophoresis, Surfactant",
author = "Masaru Kato and Yusuke Suwanai and Atsushi Shimojima and Tomofumi Santa",
year = "2012",
month = "11",
doi = "10.1002/elps.201200235",
language = "English",
volume = "33",
pages = "3339--3342",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "22",

}

TY - JOUR

T1 - A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules

AU - Kato, Masaru

AU - Suwanai, Yusuke

AU - Shimojima, Atsushi

AU - Santa, Tomofumi

PY - 2012/11

Y1 - 2012/11

N2 - The preparation of nanometer-scale pores, or nanopores, has become easy because of the progress in nanotechnology. Surfactants are promising materials for the preparation of nanostructures containing nanopores, because surfactants form many different phase structures, including cubic, micellar, and lamellar structures. We prepared a gel matrix with a cubic structure from a commercially available surfactant, polyoxyethylene(50) lauryl ether (C12EO50, Adekatol LA-50). This gel matrix had regularly arrayed nanopores between the packed spherical micelles. We used the gel to separate biomolecules by means of slab gel electrophoresis. The gel was applicable to migration of amino acids and peptides; however, larger molecules, such as proteins and single-walled carbon nanotubes, did not migrate through the gel. We concluded that the pore size was too small for the penetration of large molecules, and that only small molecules could penetrate the gel matrix. The migration mechanism of small molecules was similar to that observed in conventional gel electrophoresis. We concluded that the gel matrix prepared from surfactant is a promising matrix for migration and purification of small molecules. We also expect that the gel can be used as a nanoscale filter to trap large molecules, allowing only small molecules to pass.

AB - The preparation of nanometer-scale pores, or nanopores, has become easy because of the progress in nanotechnology. Surfactants are promising materials for the preparation of nanostructures containing nanopores, because surfactants form many different phase structures, including cubic, micellar, and lamellar structures. We prepared a gel matrix with a cubic structure from a commercially available surfactant, polyoxyethylene(50) lauryl ether (C12EO50, Adekatol LA-50). This gel matrix had regularly arrayed nanopores between the packed spherical micelles. We used the gel to separate biomolecules by means of slab gel electrophoresis. The gel was applicable to migration of amino acids and peptides; however, larger molecules, such as proteins and single-walled carbon nanotubes, did not migrate through the gel. We concluded that the pore size was too small for the penetration of large molecules, and that only small molecules could penetrate the gel matrix. The migration mechanism of small molecules was similar to that observed in conventional gel electrophoresis. We concluded that the gel matrix prepared from surfactant is a promising matrix for migration and purification of small molecules. We also expect that the gel can be used as a nanoscale filter to trap large molecules, allowing only small molecules to pass.

KW - Biomolecule

KW - Gel electrophoresis

KW - Surfactant

UR - http://www.scopus.com/inward/record.url?scp=84869845866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869845866&partnerID=8YFLogxK

U2 - 10.1002/elps.201200235

DO - 10.1002/elps.201200235

M3 - Article

C2 - 22961738

AN - SCOPUS:84869845866

VL - 33

SP - 3339

EP - 3342

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 22

ER -