Abstract
This chapter describes a target identification system in mammalian cells that enables identification of the molecular targets of bioactive compounds. This system is based on three approaches for target identification that are classified as either phenotypic profiling (indirect) or affinity beads-based pull-down (direct). The chapter describes newly developed methodologies for target identification that are MorphoBase and ChemProteoBase as indirect approaches, and photo-affinity beads as a direct approach. MorphoBase identifies molecular targets of bioactive compounds based on morphological changes of cancer cell lines induced by the compounds, while ChemProteoBase identifies molecular targets based on proteomic changes induced by the compounds. Photo-cross-linking beads are used for searching bioactive compounds-interacting target based on direct interaction of target proteins and bioactive compounds. The chapter describes the case studies of these methodologies for target identification of the bioactive compounds NPD6689/NPD8617/NPD8969, BNS-22, methyl-gerferin (M-GFN), and xanthofumol.
| Original language | English |
|---|---|
| Title of host publication | Concepts and Case Studies in Chemical Biology |
| Publisher | Wiley-Blackwell |
| Pages | 163-176 |
| Number of pages | 14 |
| Volume | 9783527336111 |
| ISBN (Electronic) | 9783527687503 |
| ISBN (Print) | 9783527336111 |
| DOIs | |
| Publication status | Published - 2014 Oct 6 |
| Externally published | Yes |
Keywords
- ChemProteoBase
- MorphoBase
- Photo-cross-linking beads
- Target identification system
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)