A transportable, inexpensive electroporator for in utero electroporation

Torsten Bullmann, Thomas Arendt, Urs Frey, Carina Hanashima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products. Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalDevelopment Growth and Differentiation
Volume57
Issue number5
DOIs
Publication statusPublished - 2015 Jun 1
Externally publishedYes

Keywords

  • Cortex
  • Electronic circuit
  • Electroporation
  • Hippocampus

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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