A transportable, inexpensive electroporator for in utero electroporation

Torsten Bullmann; Thomas Arendt; Urs Frey; Carina Hanashima

doi:10.1111/dgd.12216

A transportable, inexpensive electroporator for in utero electroporation

Torsten Bullmann^*, Thomas Arendt, Urs Frey, Carina Hanashima

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products. Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

Original language	English
Pages (from-to)	369-377
Number of pages	9
Journal	Development Growth and Differentiation
Volume	57
Issue number	5
DOIs	https://doi.org/10.1111/dgd.12216
Publication status	Published - 2015 Jun 1
Externally published	Yes

Keywords

Cortex
Electronic circuit
Electroporation
Hippocampus

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1111/dgd.12216

Cite this

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abstract = "Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products. Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.",

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AU - Bullmann, Torsten

AU - Arendt, Thomas

AU - Frey, Urs

AU - Hanashima, Carina

PY - 2015/6/1

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AB - Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products. Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

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A transportable, inexpensive electroporator for in utero electroporation

Abstract

Keywords

ASJC Scopus subject areas

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