A transportable, inexpensive electroporator for in utero electroporation

Torsten Bullmann, Thomas Arendt, Urs Frey, Carina Hanashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products. Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalDevelopment Growth and Differentiation
Volume57
Issue number5
DOIs
Publication statusPublished - 2015 Jun 1
Externally publishedYes

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Electroporation
Equipment and Supplies
Genes

Keywords

  • Cortex
  • Electronic circuit
  • Electroporation
  • Hippocampus

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

A transportable, inexpensive electroporator for in utero electroporation. / Bullmann, Torsten; Arendt, Thomas; Frey, Urs; Hanashima, Carina.

In: Development Growth and Differentiation, Vol. 57, No. 5, 01.06.2015, p. 369-377.

Research output: Contribution to journalArticle

Bullmann, Torsten ; Arendt, Thomas ; Frey, Urs ; Hanashima, Carina. / A transportable, inexpensive electroporator for in utero electroporation. In: Development Growth and Differentiation. 2015 ; Vol. 57, No. 5. pp. 369-377.
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