A Unique Dermal Dendritic Cell Subset That Skews the Immune Response toward Th2

Ryuichi Murakami, Kaori Denda-Nagai, Shin ichi Hashimoto, Shigenori Nagai, Masahira Hattori, Tatsuro Irimura

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Dendritic cell (DC) subsets in the skin and draining lymph nodes (LNs) are likely to elicit distinct immune response types. In skin and skin-draining LNs, a dermal DC subset expressing macrophage galactose-type C-type lectin 2 (MGL2/CD301b) was found distinct from migratory Langerhans cells (LCs) or CD103+ dermal DCs (dDCs). Lower expression levels of Th1-promoting and/or cross-presentation-related molecules were suggested by the transcriptome analysis and verified by the quantitative real-time PCR analysis in MGL2+ dDCs than in CD103+ dDCs. Transfer of MGL2+ dDCs but not CD103+ dDCs from FITC-sensitized mice induced a Th2-type immune response in vivo in a model of contact hypersensitivity. Targeting MGL2+ dDCs with a rat monoclonal antibody against MGL2 efficiently induced a humoral immune response with Th2-type properties, as determined by the antibody subclass. We propose that the properties of MGL2+ dDCs, are complementary to those of CD103+ dDCs and skew the immune response toward a Th2-type response.

Original languageEnglish
Article numbere73270
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - 2013 Sep 9
Externally publishedYes

Fingerprint

Langerhans Cells
dendritic cells
Skin
immune response
lymph nodes
Langerhans cells
C-Type Lectins
Fluorescein-5-isothiocyanate
Macrophages
transcriptomics
Galactose
humoral immunity
hypersensitivity
lectins
galactose
Rats
monoclonal antibodies
quantitative polymerase chain reaction
macrophages
Monoclonal Antibodies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Murakami, R., Denda-Nagai, K., Hashimoto, S. I., Nagai, S., Hattori, M., & Irimura, T. (2013). A Unique Dermal Dendritic Cell Subset That Skews the Immune Response toward Th2. PLoS One, 8(9), [e73270]. https://doi.org/10.1371/journal.pone.0073270

A Unique Dermal Dendritic Cell Subset That Skews the Immune Response toward Th2. / Murakami, Ryuichi; Denda-Nagai, Kaori; Hashimoto, Shin ichi; Nagai, Shigenori; Hattori, Masahira; Irimura, Tatsuro.

In: PLoS One, Vol. 8, No. 9, e73270, 09.09.2013.

Research output: Contribution to journalArticle

Murakami, R, Denda-Nagai, K, Hashimoto, SI, Nagai, S, Hattori, M & Irimura, T 2013, 'A Unique Dermal Dendritic Cell Subset That Skews the Immune Response toward Th2', PLoS One, vol. 8, no. 9, e73270. https://doi.org/10.1371/journal.pone.0073270
Murakami, Ryuichi ; Denda-Nagai, Kaori ; Hashimoto, Shin ichi ; Nagai, Shigenori ; Hattori, Masahira ; Irimura, Tatsuro. / A Unique Dermal Dendritic Cell Subset That Skews the Immune Response toward Th2. In: PLoS One. 2013 ; Vol. 8, No. 9.
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