A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma

Kentaro Senba, N. Kamata, K. Toyoshima, T. Yamamoto

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475 Citations (Scopus)

Abstract

From a human genomic library, we obtained six v-erbB-related DNA clones. A DNA probe prepared from one of the clones, λ107, hybridized to EcoRI fragments of 6.4 and 13 kilobase pairs of human DNA. Neither of these fragments was amplified in A431 vulva carcinoma cells, in which the gene encoding the epidermal growth factor receptor is amplified. In addition, the probe from λ107 hybridized with a single, 4.8-kilobase poly(A)+ RNA species did not react with EGF receptor mRNA. Thus, we conclude that clone λ107 represents a v-erbB-related gene (c-erbB-2) that is distinct from the EGF receptor gene. In contrast, the other five clones were shown to represent the EGF receptor gene (c-erbB-1). Partial nucleotide sequence analysis of the λ107 insert showed that this clone contained at least seven putative exons and that six of them could encode the kinase domain characteristic of protein products of the src oncogene family. Southern blot analysis showed close similarity of the restriction patterns of the rat c-erbB-2 gene and the rat neu oncogene, suggesting possible involvement of c-erbB-2 in human cancer. In fact, ~ 30-fold amplification of c-erbB-2 was observed in a human adenocarcinoma of the salivary gland.

Original languageEnglish
Pages (from-to)6497-6501
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number19
Publication statusPublished - 1985
Externally publishedYes

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erbB-1 Genes
Salivary Glands
Adenocarcinoma
Clone Cells
Epidermal Growth Factor Receptor
Oncogene Protein pp60(v-src)
erbB-2 Genes
Messenger RNA
Vulva
Genomic Library
DNA
DNA Probes
Southern Blotting
Oncogenes
Sequence Analysis
Exons
Phosphotransferases
Carcinoma
Genes
Neoplasms

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma",
abstract = "From a human genomic library, we obtained six v-erbB-related DNA clones. A DNA probe prepared from one of the clones, λ107, hybridized to EcoRI fragments of 6.4 and 13 kilobase pairs of human DNA. Neither of these fragments was amplified in A431 vulva carcinoma cells, in which the gene encoding the epidermal growth factor receptor is amplified. In addition, the probe from λ107 hybridized with a single, 4.8-kilobase poly(A)+ RNA species did not react with EGF receptor mRNA. Thus, we conclude that clone λ107 represents a v-erbB-related gene (c-erbB-2) that is distinct from the EGF receptor gene. In contrast, the other five clones were shown to represent the EGF receptor gene (c-erbB-1). Partial nucleotide sequence analysis of the λ107 insert showed that this clone contained at least seven putative exons and that six of them could encode the kinase domain characteristic of protein products of the src oncogene family. Southern blot analysis showed close similarity of the restriction patterns of the rat c-erbB-2 gene and the rat neu oncogene, suggesting possible involvement of c-erbB-2 in human cancer. In fact, ~ 30-fold amplification of c-erbB-2 was observed in a human adenocarcinoma of the salivary gland.",
author = "Kentaro Senba and N. Kamata and K. Toyoshima and T. Yamamoto",
year = "1985",
language = "English",
volume = "82",
pages = "6497--6501",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
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TY - JOUR

T1 - A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma

AU - Senba, Kentaro

AU - Kamata, N.

AU - Toyoshima, K.

AU - Yamamoto, T.

PY - 1985

Y1 - 1985

N2 - From a human genomic library, we obtained six v-erbB-related DNA clones. A DNA probe prepared from one of the clones, λ107, hybridized to EcoRI fragments of 6.4 and 13 kilobase pairs of human DNA. Neither of these fragments was amplified in A431 vulva carcinoma cells, in which the gene encoding the epidermal growth factor receptor is amplified. In addition, the probe from λ107 hybridized with a single, 4.8-kilobase poly(A)+ RNA species did not react with EGF receptor mRNA. Thus, we conclude that clone λ107 represents a v-erbB-related gene (c-erbB-2) that is distinct from the EGF receptor gene. In contrast, the other five clones were shown to represent the EGF receptor gene (c-erbB-1). Partial nucleotide sequence analysis of the λ107 insert showed that this clone contained at least seven putative exons and that six of them could encode the kinase domain characteristic of protein products of the src oncogene family. Southern blot analysis showed close similarity of the restriction patterns of the rat c-erbB-2 gene and the rat neu oncogene, suggesting possible involvement of c-erbB-2 in human cancer. In fact, ~ 30-fold amplification of c-erbB-2 was observed in a human adenocarcinoma of the salivary gland.

AB - From a human genomic library, we obtained six v-erbB-related DNA clones. A DNA probe prepared from one of the clones, λ107, hybridized to EcoRI fragments of 6.4 and 13 kilobase pairs of human DNA. Neither of these fragments was amplified in A431 vulva carcinoma cells, in which the gene encoding the epidermal growth factor receptor is amplified. In addition, the probe from λ107 hybridized with a single, 4.8-kilobase poly(A)+ RNA species did not react with EGF receptor mRNA. Thus, we conclude that clone λ107 represents a v-erbB-related gene (c-erbB-2) that is distinct from the EGF receptor gene. In contrast, the other five clones were shown to represent the EGF receptor gene (c-erbB-1). Partial nucleotide sequence analysis of the λ107 insert showed that this clone contained at least seven putative exons and that six of them could encode the kinase domain characteristic of protein products of the src oncogene family. Southern blot analysis showed close similarity of the restriction patterns of the rat c-erbB-2 gene and the rat neu oncogene, suggesting possible involvement of c-erbB-2 in human cancer. In fact, ~ 30-fold amplification of c-erbB-2 was observed in a human adenocarcinoma of the salivary gland.

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