AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea

Tatsuki Miyamoto, Akiko Yokota, Satoshi Tsuneda, Naohiro Noda

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.

    Original languageEnglish
    Article number174
    JournalToxins
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - 2016 Jun 1

    Fingerprint

    Nitrosomonas europaea
    Endoribonucleases
    RNA Cleavage
    Antitoxins
    RNA
    Chromosomes
    Assays
    Growth

    Keywords

    • MazF
    • Nitrosomonas europaea
    • Sequence-specific endoribonuclease
    • Toxin-antitoxin system

    ASJC Scopus subject areas

    • Toxicology
    • Health, Toxicology and Mutagenesis

    Cite this

    AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea. / Miyamoto, Tatsuki; Yokota, Akiko; Tsuneda, Satoshi; Noda, Naohiro.

    In: Toxins, Vol. 8, No. 6, 174, 01.06.2016.

    Research output: Contribution to journalArticle

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