Aberrant DNA methylation status in human uterine leiomyoma

Yoshiaki Yamagata, Ryo Maekawa, Hiromi Asada, Toshiaki Taketani, Isao Tamura, Hiroshi Tamura, Jun Ogane, Naka Hattori, Kunio Shiota, Norihiro Sugino

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Aberrant DNA methylation has been implicated in tumorigenesis. This study was undertaken to establish the genome-wide DNA methylation profile in uterine leiomyomas and to investigate whether DNA methylation status is altered in uterine leiomyomas. For this purpose, restriction landmark genomic scanning (RLGS) was performed on a paired sample of leiomyoma and adjacent normal myometrium. The RLGS profile revealed 29 aberrant methylation spots (10 methylated and 19 demethylated) in leiomyoma in comparison with myometrium. One of the differently methylated genomic loci was newly identified as GS20656 from the human genome sequence database. In 9 of the 10 paired samples, the DNA methylation levels of the first exon of GS20656 were significantly lower in leiomyoma than in myometrium, suggesting the existence of a genomic locus under epigenetic regulation in uterine leiomyomas. Unexpectedly, DNA methyltransferase 1 (DNMT1) and DNMT3a mRNA expression levels were higher in leiomyoma than in myometrium. These facts suggest that other epigenetic factors besides DNMT are involved in local changes of DNA methylation at genome loci. The present study indicates not only aberrant genome-wide DNA methylation status in uterine leiomyomas but also the existence of a genomic locus that is differently methylated between normal myometrium and uterine leiomyoma.

Original languageEnglish
Pages (from-to)259-267
Number of pages9
JournalMolecular Human Reproduction
Volume15
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Leiomyoma
DNA Methylation
Myometrium
Genome
Epigenomics
Methyltransferases
Human Genome
Methylation
Exons
Carcinogenesis
Databases
Messenger RNA
DNA

Keywords

  • DNA methylation
  • Leiomyoma
  • RLGS

ASJC Scopus subject areas

  • Molecular Biology
  • Embryology
  • Cell Biology
  • Genetics
  • Developmental Biology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Yamagata, Y., Maekawa, R., Asada, H., Taketani, T., Tamura, I., Tamura, H., ... Sugino, N. (2009). Aberrant DNA methylation status in human uterine leiomyoma. Molecular Human Reproduction, 15(4), 259-267. https://doi.org/10.1093/molehr/gap010

Aberrant DNA methylation status in human uterine leiomyoma. / Yamagata, Yoshiaki; Maekawa, Ryo; Asada, Hiromi; Taketani, Toshiaki; Tamura, Isao; Tamura, Hiroshi; Ogane, Jun; Hattori, Naka; Shiota, Kunio; Sugino, Norihiro.

In: Molecular Human Reproduction, Vol. 15, No. 4, 2009, p. 259-267.

Research output: Contribution to journalArticle

Yamagata, Y, Maekawa, R, Asada, H, Taketani, T, Tamura, I, Tamura, H, Ogane, J, Hattori, N, Shiota, K & Sugino, N 2009, 'Aberrant DNA methylation status in human uterine leiomyoma', Molecular Human Reproduction, vol. 15, no. 4, pp. 259-267. https://doi.org/10.1093/molehr/gap010
Yamagata Y, Maekawa R, Asada H, Taketani T, Tamura I, Tamura H et al. Aberrant DNA methylation status in human uterine leiomyoma. Molecular Human Reproduction. 2009;15(4):259-267. https://doi.org/10.1093/molehr/gap010
Yamagata, Yoshiaki ; Maekawa, Ryo ; Asada, Hiromi ; Taketani, Toshiaki ; Tamura, Isao ; Tamura, Hiroshi ; Ogane, Jun ; Hattori, Naka ; Shiota, Kunio ; Sugino, Norihiro. / Aberrant DNA methylation status in human uterine leiomyoma. In: Molecular Human Reproduction. 2009 ; Vol. 15, No. 4. pp. 259-267.
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