Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils

Keiichi Higuchi, Kumiko Kogishi, Jing Wang, Chen Xia, Takuya Chiba, Takatoshi Matsushita, Masanori Hosokawa

Research output: Contribution to journalArticle

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Abstract

Apolipoprotein A-II (apoA-II), the major apoprotein of serum high-density lipoprotein, is deposited as amyloid fibrils (AApoAII) in murine senile amyloidosis. We have identified and purified a more basic amyloid protein from old-mouse liver. N-terminal sequencing of the protein revealed that the pro-segment of five amino acid residues (Ala-Leu-Val-Lys-Arg) extended from the N-terminal glutamine residue of mature apoA-II protein. MS analysis revealed the deposit of intact pro-apoA-II protein (molecular mass 9319 Da). Antiserum was prepared for staining of the AApoAII amyloid deposition. The relative abundance of pro-apoA-II to mature apoA-II in the amyloid-fibril fraction isolated from livers of mice with severe amyloidosis was 14.1%. The similar abundance of pro-apoA-II in the amyloid fibril fraction from the spleen (16.3%) suggested that deposited pro-apoA-II originated from the blood. The concentration of pro-apoA-II was much lower in the serum (1.5% of mature apoA-II) than in the amyloid-fibril fraction. There was no difference in the content of pro-apoA-II between the amyloidogenetic R1.P1-Apoa2(c) and amyloid-resistant SAMR1 strains at the age of 3 months. The abundance of pro-apoA-II in the amyloid-fibril fraction compared with the serum suggested that it plays a key role in the initialization of mouse senile amyloidosis.

Original languageEnglish
Pages (from-to)653-659
Number of pages7
JournalBiochemical Journal
Volume325
Issue number3
Publication statusPublished - 1997 Aug 1
Externally publishedYes

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Apolipoprotein A-II
Amyloid
Amyloidosis
Liver
Serum
Amyloidogenic Proteins
Proteins
Apoproteins
Protein Sequence Analysis
Molecular mass
HDL Lipoproteins
Glutamine
Immune Sera
Blood
Spleen
Deposits

ASJC Scopus subject areas

  • Biochemistry

Cite this

Higuchi, K., Kogishi, K., Wang, J., Xia, C., Chiba, T., Matsushita, T., & Hosokawa, M. (1997). Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils. Biochemical Journal, 325(3), 653-659.

Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils. / Higuchi, Keiichi; Kogishi, Kumiko; Wang, Jing; Xia, Chen; Chiba, Takuya; Matsushita, Takatoshi; Hosokawa, Masanori.

In: Biochemical Journal, Vol. 325, No. 3, 01.08.1997, p. 653-659.

Research output: Contribution to journalArticle

Higuchi, K, Kogishi, K, Wang, J, Xia, C, Chiba, T, Matsushita, T & Hosokawa, M 1997, 'Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils', Biochemical Journal, vol. 325, no. 3, pp. 653-659.
Higuchi K, Kogishi K, Wang J, Xia C, Chiba T, Matsushita T et al. Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils. Biochemical Journal. 1997 Aug 1;325(3):653-659.
Higuchi, Keiichi ; Kogishi, Kumiko ; Wang, Jing ; Xia, Chen ; Chiba, Takuya ; Matsushita, Takatoshi ; Hosokawa, Masanori. / Accumulation of pro-apolipoprotein A-II in mouse senile amyloid fibrils. In: Biochemical Journal. 1997 ; Vol. 325, No. 3. pp. 653-659.
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