Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells

Mikio Takagi, Masaya Ikegawa, Takashi Shimada, Susumu Ishikawa, Mihoko Kajita, Takeshi Maruyama, Tomoko Kamasaki, Yasuyuki Fujita

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.

Original languageEnglish
JournalGenes to Cells
DOIs
Publication statusAccepted/In press - 2018 Jan 1
Externally publishedYes

Fingerprint

Myosin Type II
Spectrin
Epithelial Cells
Epithelium
Preventive Medicine
Coculture Techniques
Neoplasms
Carrier Proteins
Carcinogenesis

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. / Takagi, Mikio; Ikegawa, Masaya; Shimada, Takashi; Ishikawa, Susumu; Kajita, Mihoko; Maruyama, Takeshi; Kamasaki, Tomoko; Fujita, Yasuyuki.

In: Genes to Cells, 01.01.2018.

Research output: Contribution to journalArticle

Takagi, Mikio ; Ikegawa, Masaya ; Shimada, Takashi ; Ishikawa, Susumu ; Kajita, Mihoko ; Maruyama, Takeshi ; Kamasaki, Tomoko ; Fujita, Yasuyuki. / Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. In: Genes to Cells. 2018.
@article{55fdec989f314659b9f680e919f14d17,
title = "Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells",
abstract = "At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.",
author = "Mikio Takagi and Masaya Ikegawa and Takashi Shimada and Susumu Ishikawa and Mihoko Kajita and Takeshi Maruyama and Tomoko Kamasaki and Yasuyuki Fujita",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/gtc.12643",
language = "English",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells

AU - Takagi, Mikio

AU - Ikegawa, Masaya

AU - Shimada, Takashi

AU - Ishikawa, Susumu

AU - Kajita, Mihoko

AU - Maruyama, Takeshi

AU - Kamasaki, Tomoko

AU - Fujita, Yasuyuki

PY - 2018/1/1

Y1 - 2018/1/1

N2 - At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.

AB - At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.

UR - http://www.scopus.com/inward/record.url?scp=85054167406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054167406&partnerID=8YFLogxK

U2 - 10.1111/gtc.12643

DO - 10.1111/gtc.12643

M3 - Article

C2 - 30175422

AN - SCOPUS:85054167406

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

ER -