Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters

Shinji Fukuda, Hidehiro Toh, Todd D. Taylor, Hiroshi Ohno, Masahira Hattori

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The human gut harbors a large and diverse community of commensal bacteria. Among them, Bifidobacterium is known to exhibit various probiotic effects including protection of hosts from infectious diseases. We recently discovered that genes encoding an ATPbinding- cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting gnotobiotic mice from death induced by enterohemorrhagic Escherichia coli O157:H7. We elucidated the molecular mechanism on lethal infection in mice associated with several bifidobacterial strains by a multiomics approach combining genomics, transcriptomics and metabolomics. The combined data clearly show that acetate produced by protective bifidobacteria acts in vivo to promote defense functions of the host epithelial cells and thereby protects the host from lethal infection. As demonstrated here, our multi-omics approach provides a powerful strategy for evaluation of host-microbial interactions in the complex gut ecosystem.

Original languageEnglish
JournalGut Microbes
Volume3
Issue number5
DOIs
Publication statusPublished - 2012 Jan 1
Externally publishedYes

Fingerprint

Bifidobacterium
Acetates
Carbohydrates
Infection
Microbial Interactions
Germ-Free Life
Enterohemorrhagic Escherichia coli
Escherichia coli O157
Metabolomics
Probiotics
Genomics
Communicable Diseases
Ecosystem
Epithelial Cells
Bacteria
Genes

Keywords

  • Acetate
  • Bifidobacteria
  • Carbohydrate transporter
  • EHEC
  • Genome
  • Gnotobiotic mouse
  • Gut microbiome
  • Metabolome
  • Omics
  • Probiotics

ASJC Scopus subject areas

  • Microbiology
  • Gastroenterology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters. / Fukuda, Shinji; Toh, Hidehiro; Taylor, Todd D.; Ohno, Hiroshi; Hattori, Masahira.

In: Gut Microbes, Vol. 3, No. 5, 01.01.2012.

Research output: Contribution to journalArticle

Fukuda, Shinji ; Toh, Hidehiro ; Taylor, Todd D. ; Ohno, Hiroshi ; Hattori, Masahira. / Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters. In: Gut Microbes. 2012 ; Vol. 3, No. 5.
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