Acquisition of neuronal proteins during differentiation of NG108-15 cells

Takuro Tojima, Yukako Yamane, Masayuki Takahashi, Etsuro Ito

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The differentiated type of neuroblastomaxglioma hybrid cell line, NG108-15, has widely been used in in vitro studies instead of primary-cultured neurons. Here we examined whether NG108-15 cells can be used as a model for studying the neuronal differentiation process. We compared the expression of neuronal proteins (neurofilament 200 (NF200), phosphorylated-NF200 (p-NF200), microtubule associated protein 2, synaptophysin, syntaxin 1, choline acetyltransferase, and acetylcholinesterase (AChE)) and a glial protein (vimentin) between undifferentiated and differentiated NG108-15 cells by immunocytochemistry and immunoblot analysis. The expression of all neuronal proteins, with the exception of NF200 and p-NF200, was positive in differentiated cells, but almost negative in undifferentiated cells. On the other hand, cytoskeletal intermediate filaments (NF200 and p-NF200) for neurons and that (vimentin) for glia were present in both undifferentiated and differentiated cells. Furthermore, a high expression of AChE mRNA was confirmed in differentiated cells by reverse transcription-PCR analysis. Our results showed that even though the expression of cytoskeletal filaments does not change during differentiation of NG108-15 cells, these cells during differentiation can serve as an appropriate tool for investigating and understanding the mechanisms involved in neuronal development and differentiation. Copyright (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalNeuroscience Research
Volume37
Issue number2
DOIs
Publication statusPublished - 2000 Jun
Externally publishedYes

Fingerprint

Proteins
Vimentin
Acetylcholinesterase
Cytoskeleton
Neuroglia
Syntaxin 1
Neurons
Synaptophysin
Microtubule-Associated Proteins
Choline O-Acetyltransferase
Intermediate Filaments
Hybrid Cells
Neurosciences
Reverse Transcription
Cell Differentiation
Japan
Immunohistochemistry
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Differentiation
  • Glial protein
  • Immunoblot analysis
  • Immunocytochemistry
  • Neuronal protein
  • NG108-15

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Acquisition of neuronal proteins during differentiation of NG108-15 cells. / Tojima, Takuro; Yamane, Yukako; Takahashi, Masayuki; Ito, Etsuro.

In: Neuroscience Research, Vol. 37, No. 2, 06.2000, p. 153-161.

Research output: Contribution to journalArticle

Tojima, Takuro ; Yamane, Yukako ; Takahashi, Masayuki ; Ito, Etsuro. / Acquisition of neuronal proteins during differentiation of NG108-15 cells. In: Neuroscience Research. 2000 ; Vol. 37, No. 2. pp. 153-161.
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