Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A

E. Yokota, N. Imamichi, Motoki Tominaga, T. Shimmen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In root hair cells of Limnobium stoloniferum, a protein phosphatase inhibitor, calyculin A (CA), at concentrations higher than 50 nM inhibits cytoplasmic streaming and induces remarkable morphological changes in the cytoplasm: the transvacuolar strands disperse and spherical cytoplasmic bodies emerge. The mechanism of the morphological changes of the cytoplasm induced by CA was studied by pharmacological analyses. The formation of spherical bodies in cells treated with CA was suppressed by the actin-depolymerizing and -fragmenting drugs latrunculin B and cytochalasin D at concentrations higher than 100 nM and 5 μM, respectively. In contrast, 100 μM propyzamide, a microtubule-depolymerizing drug, did not affect the formation of spherical bodies by CA. Interestingly, 60 mM 2,3-butanedione monoxime, an inhibitor of myosin, also suppressed the CA-induced formation of cytoplasmic spherical bodies. These results indicate that the actin cytoskeleton is intimately involved in the morphological changes of the cytoplasm induced by CA.

Original languageEnglish
Pages (from-to)184-193
Number of pages10
JournalProtoplasma
Volume213
Issue number3-4
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Phosphoprotein Phosphatases
root hairs
microfilaments
Actin Cytoskeleton
cytoplasm
Limnobium
propyzamide
cytoplasmic streaming
cytochalasin D
drugs
Cytoplasm
proteins
cells
myosin
microtubules
actin
Hydrocharitaceae
Cytoplasmic Streaming
Cytochalasin D
Myosins

Keywords

  • Actin
  • Calyculin A
  • Cytoplasmic streaming
  • Phosphatases
  • Root hair cell
  • Transvacuolar strand

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A. / Yokota, E.; Imamichi, N.; Tominaga, Motoki; Shimmen, T.

In: Protoplasma, Vol. 213, No. 3-4, 2000, p. 184-193.

Research output: Contribution to journalArticle

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