Abstract
A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2% sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.
| Original language | English |
|---|---|
| Article number | 002714 |
| Pages (from-to) | 1584-1590 |
| Number of pages | 7 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 68 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2018 May 1 |
| Externally published | Yes |
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Keywords
- Actinomadura barringtoniae sp. nov
- Endophytic actinomycete
- Indian oak tree
- New species
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Actinomadura barringtoniae sp. Nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn. / Rachniyom, Hathairat; Matsumoto, Atsuko; Inahashi, Yuki; Take, Akira; Takahashi, Yoko; Thamchaipenet, Arinthip.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 5, 002714, 01.05.2018, p. 1584-1590.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Actinomadura barringtoniae sp. Nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn
AU - Rachniyom, Hathairat
AU - Matsumoto, Atsuko
AU - Inahashi, Yuki
AU - Take, Akira
AU - Takahashi, Yoko
AU - Thamchaipenet, Arinthip
PY - 2018/5/1
Y1 - 2018/5/1
N2 - A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2% sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.
AB - A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2% sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.
KW - Actinomadura barringtoniae sp. nov
KW - Endophytic actinomycete
KW - Indian oak tree
KW - New species
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UR - http://www.scopus.com/inward/citedby.url?scp=85046542764&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002714
DO - 10.1099/ijsem.0.002714
M3 - Article
C2 - 29543147
AN - SCOPUS:85046542764
VL - 68
SP - 1584
EP - 1590
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 5
M1 - 002714
ER -