Activation of macrophages by a laccase-polymerized polyphenol is dependent on phosphorylation of Rac1

Katsuya Tajima, Satoshi Akanuma, Akiko Matsumoto-Akanuma, Daisuke Yamanaka, Ken ichi Ishibashi, Yoshiyuki Adachi, Naohito Ohno

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    Abstract

    Various physiologically active effects of polymerized polyphenols have been reported. In this study, we synthesized a polymerized polyphenol (mL2a-pCA) by polymerizing caffeic acid using mutant Agaricus brasiliensis laccase and analyzed its physiological activity and mechanism of action. We found that mL2a-pCA induced morphological changes and the production of cytokines and chemokines in C3H/HeN mouse-derived resident peritoneal macrophages in vitro. The mechanisms of action of polymerized polyphenols on in vitro mouse resident peritoneal cells have not been characterized in detail previously. Herein, we report that the mL2a-pCA-induced production of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in C3H/HeN mouse-derived resident peritoneal cells was inhibited by treatment with the Rac1 inhibitor NSC23766 trihydrochloride. In addition, we found that mL2a-pCA activated the phosphorylation Rac1. Taken together, the results show that mL2a-pCA induced macrophage activation via Rac1 phosphorylation-dependent pathways.

    Original languageEnglish
    Pages (from-to)2209-2213
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume495
    Issue number3
    DOIs
    Publication statusPublished - 2018 Jan 15

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    Keywords

    • Agaricus brasiliensis
    • Laccase
    • Macrophage
    • Polymerized polyphenol
    • Rac1

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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