Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice

Ren Yong Li, Satoshi Baba, Isao Kosugi, Yoshifumi Arai, Hideya Kawasaki, Yuichiro Shinmura, Shinichi Sakakibara, Hideyuki Okano, Yoshihiro Tsutsui

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cytomegalovirus (CMV) is the most common infectious cause of congenital anomalies of the CNS in humans. We recently reported that the murine cytomegalovirus (MCMV) immediate-early (IE) gene promoter directs astrocyte-specific expression in adult transgenic mice. In the present study, we analyzed the activation of the MCMV IE promoter in developing transgenic mouse brains and compared the activation with that of the Musashi 1 (Msi1) gene, which is expressed in neural progenitor cells, including neural stem cells. During the early phase of neurogenesis, the transgene was expressed predominantly in endothelial cells of the vessels, but not in neuroepithelial cells in which Msi1 was expressed. During later stages of gestation, expression of the transgene was largely restricted to the ventricular zone (VZ) in the CNS, similar to the expression of Msi1. In neurosphere cultures from transgenic embryos in the late phase of neurogenesis, the transgene was expressed in some cells of neurospheres expressing Msi1 and nestin. In neural precursor cells induced to differentiate from stem cells, expression of the transgene was detected in glial progenitor cells, expressing GFAP, nestin, and Msi1, but not in cells expressing MAP2 or MAG. In postnatal development, persistent expression of the transgene was observed in astrocyte lineage cells as was Msi1. These spatiotemporal changes of the MCMV IE promoter activity during development of transgenic mice correlated with susceptible sites in congenital HCMV infection. Moreover, this transgenic mouse model may provide useful model for analysis of the regulation of the switching of neuronal and astrocyte differentiation, and the maintenance of the astrocyte lineage.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalGLIA
Volume35
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Muromegalovirus
Transgenes
Neuroglia
Transgenic Mice
Stem Cells
Astrocytes
Nestin
Neurogenesis
Neuroepithelial Cells
Immediate-Early Genes
Neural Stem Cells
Cytomegalovirus
Embryonic Structures
Endothelial Cells
Maintenance
Pregnancy
Brain
Infection
Genes

Keywords

  • Brain abnormalities
  • Congenital infection
  • Glial differentiation
  • Musashi1 gene
  • Neural stem cell

ASJC Scopus subject areas

  • Immunology

Cite this

Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice. / Li, Ren Yong; Baba, Satoshi; Kosugi, Isao; Arai, Yoshifumi; Kawasaki, Hideya; Shinmura, Yuichiro; Sakakibara, Shinichi; Okano, Hideyuki; Tsutsui, Yoshihiro.

In: GLIA, Vol. 35, No. 1, 2001, p. 41-52.

Research output: Contribution to journalArticle

Li, Ren Yong ; Baba, Satoshi ; Kosugi, Isao ; Arai, Yoshifumi ; Kawasaki, Hideya ; Shinmura, Yuichiro ; Sakakibara, Shinichi ; Okano, Hideyuki ; Tsutsui, Yoshihiro. / Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice. In: GLIA. 2001 ; Vol. 35, No. 1. pp. 41-52.
@article{5c9bd64390854f40a7f954b9839b74f6,
title = "Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice",
abstract = "Cytomegalovirus (CMV) is the most common infectious cause of congenital anomalies of the CNS in humans. We recently reported that the murine cytomegalovirus (MCMV) immediate-early (IE) gene promoter directs astrocyte-specific expression in adult transgenic mice. In the present study, we analyzed the activation of the MCMV IE promoter in developing transgenic mouse brains and compared the activation with that of the Musashi 1 (Msi1) gene, which is expressed in neural progenitor cells, including neural stem cells. During the early phase of neurogenesis, the transgene was expressed predominantly in endothelial cells of the vessels, but not in neuroepithelial cells in which Msi1 was expressed. During later stages of gestation, expression of the transgene was largely restricted to the ventricular zone (VZ) in the CNS, similar to the expression of Msi1. In neurosphere cultures from transgenic embryos in the late phase of neurogenesis, the transgene was expressed in some cells of neurospheres expressing Msi1 and nestin. In neural precursor cells induced to differentiate from stem cells, expression of the transgene was detected in glial progenitor cells, expressing GFAP, nestin, and Msi1, but not in cells expressing MAP2 or MAG. In postnatal development, persistent expression of the transgene was observed in astrocyte lineage cells as was Msi1. These spatiotemporal changes of the MCMV IE promoter activity during development of transgenic mice correlated with susceptible sites in congenital HCMV infection. Moreover, this transgenic mouse model may provide useful model for analysis of the regulation of the switching of neuronal and astrocyte differentiation, and the maintenance of the astrocyte lineage.",
keywords = "Brain abnormalities, Congenital infection, Glial differentiation, Musashi1 gene, Neural stem cell",
author = "Li, {Ren Yong} and Satoshi Baba and Isao Kosugi and Yoshifumi Arai and Hideya Kawasaki and Yuichiro Shinmura and Shinichi Sakakibara and Hideyuki Okano and Yoshihiro Tsutsui",
year = "2001",
doi = "10.1002/glia.1069",
language = "English",
volume = "35",
pages = "41--52",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice

AU - Li, Ren Yong

AU - Baba, Satoshi

AU - Kosugi, Isao

AU - Arai, Yoshifumi

AU - Kawasaki, Hideya

AU - Shinmura, Yuichiro

AU - Sakakibara, Shinichi

AU - Okano, Hideyuki

AU - Tsutsui, Yoshihiro

PY - 2001

Y1 - 2001

N2 - Cytomegalovirus (CMV) is the most common infectious cause of congenital anomalies of the CNS in humans. We recently reported that the murine cytomegalovirus (MCMV) immediate-early (IE) gene promoter directs astrocyte-specific expression in adult transgenic mice. In the present study, we analyzed the activation of the MCMV IE promoter in developing transgenic mouse brains and compared the activation with that of the Musashi 1 (Msi1) gene, which is expressed in neural progenitor cells, including neural stem cells. During the early phase of neurogenesis, the transgene was expressed predominantly in endothelial cells of the vessels, but not in neuroepithelial cells in which Msi1 was expressed. During later stages of gestation, expression of the transgene was largely restricted to the ventricular zone (VZ) in the CNS, similar to the expression of Msi1. In neurosphere cultures from transgenic embryos in the late phase of neurogenesis, the transgene was expressed in some cells of neurospheres expressing Msi1 and nestin. In neural precursor cells induced to differentiate from stem cells, expression of the transgene was detected in glial progenitor cells, expressing GFAP, nestin, and Msi1, but not in cells expressing MAP2 or MAG. In postnatal development, persistent expression of the transgene was observed in astrocyte lineage cells as was Msi1. These spatiotemporal changes of the MCMV IE promoter activity during development of transgenic mice correlated with susceptible sites in congenital HCMV infection. Moreover, this transgenic mouse model may provide useful model for analysis of the regulation of the switching of neuronal and astrocyte differentiation, and the maintenance of the astrocyte lineage.

AB - Cytomegalovirus (CMV) is the most common infectious cause of congenital anomalies of the CNS in humans. We recently reported that the murine cytomegalovirus (MCMV) immediate-early (IE) gene promoter directs astrocyte-specific expression in adult transgenic mice. In the present study, we analyzed the activation of the MCMV IE promoter in developing transgenic mouse brains and compared the activation with that of the Musashi 1 (Msi1) gene, which is expressed in neural progenitor cells, including neural stem cells. During the early phase of neurogenesis, the transgene was expressed predominantly in endothelial cells of the vessels, but not in neuroepithelial cells in which Msi1 was expressed. During later stages of gestation, expression of the transgene was largely restricted to the ventricular zone (VZ) in the CNS, similar to the expression of Msi1. In neurosphere cultures from transgenic embryos in the late phase of neurogenesis, the transgene was expressed in some cells of neurospheres expressing Msi1 and nestin. In neural precursor cells induced to differentiate from stem cells, expression of the transgene was detected in glial progenitor cells, expressing GFAP, nestin, and Msi1, but not in cells expressing MAP2 or MAG. In postnatal development, persistent expression of the transgene was observed in astrocyte lineage cells as was Msi1. These spatiotemporal changes of the MCMV IE promoter activity during development of transgenic mice correlated with susceptible sites in congenital HCMV infection. Moreover, this transgenic mouse model may provide useful model for analysis of the regulation of the switching of neuronal and astrocyte differentiation, and the maintenance of the astrocyte lineage.

KW - Brain abnormalities

KW - Congenital infection

KW - Glial differentiation

KW - Musashi1 gene

KW - Neural stem cell

UR - http://www.scopus.com/inward/record.url?scp=0034958910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034958910&partnerID=8YFLogxK

U2 - 10.1002/glia.1069

DO - 10.1002/glia.1069

M3 - Article

C2 - 11424191

AN - SCOPUS:0034958910

VL - 35

SP - 41

EP - 52

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 1

ER -