Activation of the G protein Gq/11 through tyrosine phosphorylation of the α subunit

Hisashi Umemori, Takafumi Inoue, Shoen Kume, Naohiro Sekiyama, Motoshi Nagao, Hiroshi Itoh, Shigetada Nakanishi, Katsuhiko Mikoshiba, Tadashi Yamamoto

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Various receptors coupled to the heterotrimeric guanine nucleotide- binding protein Gq/11 stimulate formation of inositol-1,4,5-trisphosphate (IP3). Activation of these receptors also induces protein tyrosine phosphorylation. Formation of IP3 in response to stimulated receptors that couple to Gq/11 was blocked by protein tyrosine kinase inhibitors. These inhibitors appeared to act before activation of Gq/11. Moreover, stimulation of receptors coupled to Gq/11 induced phosphorylation on a tyrosine residue (Tyr356) of the Gα(q/11) subunit, and this tyrosine phosphorylation event was essential for Gq/11 activation. Tyrosine phosphorylation of Gα(q/11) induced changes in its interaction with receptors. Therefore, tyrosine phosphorylation of Gα(q/11) appears to regulate the activation of Gq/11 protein.

Original languageEnglish
Pages (from-to)1878-1881
Number of pages4
JournalScience
Volume276
Issue number5320
DOIs
Publication statusPublished - 1997 Jun 20
Externally publishedYes

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Gq-G11 GTP-Binding Protein alpha Subunits
Tyrosine
Phosphorylation
Inositol 1,4,5-Trisphosphate
Guanine Nucleotides
Protein Kinase Inhibitors
Protein-Tyrosine Kinases
Carrier Proteins
Proteins

ASJC Scopus subject areas

  • General

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Umemori, H., Inoue, T., Kume, S., Sekiyama, N., Nagao, M., Itoh, H., ... Yamamoto, T. (1997). Activation of the G protein Gq/11 through tyrosine phosphorylation of the α subunit. Science, 276(5320), 1878-1881. https://doi.org/10.1126/science.276.5320.1878

Activation of the G protein Gq/11 through tyrosine phosphorylation of the α subunit. / Umemori, Hisashi; Inoue, Takafumi; Kume, Shoen; Sekiyama, Naohiro; Nagao, Motoshi; Itoh, Hiroshi; Nakanishi, Shigetada; Mikoshiba, Katsuhiko; Yamamoto, Tadashi.

In: Science, Vol. 276, No. 5320, 20.06.1997, p. 1878-1881.

Research output: Contribution to journalArticle

Umemori, H, Inoue, T, Kume, S, Sekiyama, N, Nagao, M, Itoh, H, Nakanishi, S, Mikoshiba, K & Yamamoto, T 1997, 'Activation of the G protein Gq/11 through tyrosine phosphorylation of the α subunit', Science, vol. 276, no. 5320, pp. 1878-1881. https://doi.org/10.1126/science.276.5320.1878
Umemori, Hisashi ; Inoue, Takafumi ; Kume, Shoen ; Sekiyama, Naohiro ; Nagao, Motoshi ; Itoh, Hiroshi ; Nakanishi, Shigetada ; Mikoshiba, Katsuhiko ; Yamamoto, Tadashi. / Activation of the G protein Gq/11 through tyrosine phosphorylation of the α subunit. In: Science. 1997 ; Vol. 276, No. 5320. pp. 1878-1881.
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