Activation of vitronectin (serum spreading factor) binding of heparin by denaturing agents

Masao Hayashi, Takao Akama, Ichiro Kono, Heihachiro Kashiwagi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Vitronectin (serum spreading factor), a cell-adhesive glycoprotein present in mammalian serum, has previously been the subject of conflicting reports concerning its binding to heparin. Vitronectin purified from human plasma does not bind to heparin under physiological conditions, but it does so after treatment with denaturing agents including 8 m urea or 6 m guanidine-HCl, or heating at 100°C for 5 min. These treatments seem to expose a heparin-binding site in vitronectin; this finding thus resolves the conflicts concerning this function.

Original languageEnglish
Pages (from-to)1135-1138
Number of pages4
JournalJournal of Biochemistry
Volume98
Issue number4
Publication statusPublished - 1985 Oct
Externally publishedYes

Fingerprint

hyaluronate lyase
Heparin
Vitronectin
Activation
Chemical activation
Serum
Plasma (human)
Glycoproteins
Glycoprotein
Guanidine
Adhesives
Binding sites
Urea
Heating
Resolve
Plasma
Binding Sites
Cell
Factors

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Activation of vitronectin (serum spreading factor) binding of heparin by denaturing agents. / Hayashi, Masao; Akama, Takao; Kono, Ichiro; Kashiwagi, Heihachiro.

In: Journal of Biochemistry, Vol. 98, No. 4, 10.1985, p. 1135-1138.

Research output: Contribution to journalArticle

Hayashi, Masao ; Akama, Takao ; Kono, Ichiro ; Kashiwagi, Heihachiro. / Activation of vitronectin (serum spreading factor) binding of heparin by denaturing agents. In: Journal of Biochemistry. 1985 ; Vol. 98, No. 4. pp. 1135-1138.
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