Activin A increases the number of follicle-stimulating hormone cells in anterior pituitary cultures

Tetsuro Katayama, Kunio Shiota, Michio Takahashi

Research output: Contribution to journalArticle

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Abstract

The mode of action of activin A on the anterior pituitary gland (AP) was investigated using primary cultured cells. The AP cells were cultured with activin A (1 ng/ml) at various cell densities for 24-96 h, and further incubated for 3 h with activin A-free medium. When the cells were pretreated with activin A for 48 h, follicle-stimulating hormone (FSH) secretion during the following 3-h incubation was increased only at a density of 1 × 105 cells/200 mm2, but not at 2 × 105 or 4 × 105 cells/200 mm2. A longer pretreatment (96 h) was required in order to induce this response at a density of 2 × 105 cells/200 mm2. Luteinizing hormone (LH) secretion was not affected by activin A. Thus, the FSH secretory activity of the primary culture of AP was stimulated by activin A in a cell density-dependent manner. Furthermore, it was found that treatment with activin A (10 ng/ml) for 72 h increased the number of immunoreactive FSH cells by 25-41%, and that these newly induced FSH cells were low responders to gonadotropin-releasing hormone stimulation. The proportions of immunoreactive LH, thyroid-stimulating hormone, prolactin or growth hormone cells were not affected. From these results, we conclude that activin A increases FSH secretion by changing the cell population of pituitary gonadotropes.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume69
Issue number2-3
DOIs
Publication statusPublished - 1990 Mar 5
Externally publishedYes

Fingerprint

Follicle Stimulating Hormone
Anterior Pituitary Gland
Luteinizing Hormone
Cultured Cells
Cell Count
Cells
activin A
Thyrotropin
Gonadotropin-Releasing Hormone
Prolactin
Growth Hormone
Population

Keywords

  • Activin A
  • Anterior pituitary
  • Cell density
  • Cell number

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Activin A increases the number of follicle-stimulating hormone cells in anterior pituitary cultures. / Katayama, Tetsuro; Shiota, Kunio; Takahashi, Michio.

In: Molecular and Cellular Endocrinology, Vol. 69, No. 2-3, 05.03.1990, p. 179-185.

Research output: Contribution to journalArticle

Katayama, Tetsuro ; Shiota, Kunio ; Takahashi, Michio. / Activin A increases the number of follicle-stimulating hormone cells in anterior pituitary cultures. In: Molecular and Cellular Endocrinology. 1990 ; Vol. 69, No. 2-3. pp. 179-185.
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