TY - JOUR
T1 - Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics
AU - Bannai, Hiroko
AU - Lévi, Sabine
AU - Schweizer, Claude
AU - Inoue, Takafumi
AU - Launey, Thomas
AU - Racine, Victor
AU - Sibarita, Jean Baptiste
AU - Mikoshiba, Katsuhiko
AU - Triller, Antoine
N1 - Funding Information:
This work was supported by the Institut National de la Science et de la Recherche Médicale, the Ministère de la Recherche, the Fondation pour la Recherche Médicale, the Agence National de la Recherche (ANR05-Neuro 043-01), the Toyobo Biotechnology Foundation, the Japan Society for the Promotion of Science, the Special Postdoctoral Researchers Program in RIKEN, and KAKENHI (20700300). We thank Steve Moss for GABA A Rβ3 antibody, Richard Miles and Christian Specht for reading the manuscript, and Anne Feltz for nifedipine. We also thank M. Dahan, M.-V. Ehrensperger, S. Bonneau, and T. Kim for the data analysis program and N. Morimura and M. Enomoto for biochemistry.
PY - 2009/6/11
Y1 - 2009/6/11
N2 - An activity-dependent change in synaptic efficacy is a central tenet in learning, memory, and pathological states of neuronal excitability. The lateral diffusion dynamics of neurotransmitter receptors are one of the important parameters regulating synaptic efficacy. We report here that neuronal activity modifies diffusion properties of type-A GABA receptors (GABAAR) in cultured hippocampal neurons: enhanced excitatory synaptic activity decreases the cluster size of GABAARs and reduces GABAergic mIPSC. Single-particle tracking of the GABAAR γ2 subunit labeled with quantum dots reveals that the diffusion coefficient and the synaptic confinement domain size of GABAAR increases in parallel with neuronal activity, depending on Ca2+ influx and calcineurin activity. These results indicate that GABAAR diffusion dynamics are directly linked to rapid and plastic modifications of inhibitory synaptic transmission in response to changes in intracellular Ca2+ concentration. This transient activity-dependent reduction of inhibition would favor the onset of LTP during conditioning.
AB - An activity-dependent change in synaptic efficacy is a central tenet in learning, memory, and pathological states of neuronal excitability. The lateral diffusion dynamics of neurotransmitter receptors are one of the important parameters regulating synaptic efficacy. We report here that neuronal activity modifies diffusion properties of type-A GABA receptors (GABAAR) in cultured hippocampal neurons: enhanced excitatory synaptic activity decreases the cluster size of GABAARs and reduces GABAergic mIPSC. Single-particle tracking of the GABAAR γ2 subunit labeled with quantum dots reveals that the diffusion coefficient and the synaptic confinement domain size of GABAAR increases in parallel with neuronal activity, depending on Ca2+ influx and calcineurin activity. These results indicate that GABAAR diffusion dynamics are directly linked to rapid and plastic modifications of inhibitory synaptic transmission in response to changes in intracellular Ca2+ concentration. This transient activity-dependent reduction of inhibition would favor the onset of LTP during conditioning.
KW - CELLBIO
KW - MOLNEURO
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U2 - 10.1016/j.neuron.2009.04.023
DO - 10.1016/j.neuron.2009.04.023
M3 - Article
C2 - 19524526
AN - SCOPUS:66749130829
SN - 0896-6273
VL - 62
SP - 670
EP - 682
JO - Neuron
JF - Neuron
IS - 5
ER -