Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics

Hiroko Bannai, Sabine Lévi, Claude Schweizer, Takafumi Inoue, Thomas Launey, Victor Racine, Jean Baptiste Sibarita, Katsuhiko Mikoshiba, Antoine Triller

    Research output: Contribution to journalArticle

    184 Citations (Scopus)

    Abstract

    An activity-dependent change in synaptic efficacy is a central tenet in learning, memory, and pathological states of neuronal excitability. The lateral diffusion dynamics of neurotransmitter receptors are one of the important parameters regulating synaptic efficacy. We report here that neuronal activity modifies diffusion properties of type-A GABA receptors (GABAAR) in cultured hippocampal neurons: enhanced excitatory synaptic activity decreases the cluster size of GABAARs and reduces GABAergic mIPSC. Single-particle tracking of the GABAAR γ2 subunit labeled with quantum dots reveals that the diffusion coefficient and the synaptic confinement domain size of GABAAR increases in parallel with neuronal activity, depending on Ca2+ influx and calcineurin activity. These results indicate that GABAAR diffusion dynamics are directly linked to rapid and plastic modifications of inhibitory synaptic transmission in response to changes in intracellular Ca2+ concentration. This transient activity-dependent reduction of inhibition would favor the onset of LTP during conditioning.

    Original languageEnglish
    Pages (from-to)670-682
    Number of pages13
    JournalNeuron
    Volume62
    Issue number5
    DOIs
    Publication statusPublished - 2009 Jun 11

    Fingerprint

    Synaptic Transmission
    Quantum Dots
    Neurotransmitter Receptor
    Calcineurin
    GABA-A Receptors
    Plastics
    Learning
    Neurons

    Keywords

    • CELLBIO
    • MOLNEURO

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Bannai, H., Lévi, S., Schweizer, C., Inoue, T., Launey, T., Racine, V., ... Triller, A. (2009). Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics. Neuron, 62(5), 670-682. https://doi.org/10.1016/j.neuron.2009.04.023

    Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics. / Bannai, Hiroko; Lévi, Sabine; Schweizer, Claude; Inoue, Takafumi; Launey, Thomas; Racine, Victor; Sibarita, Jean Baptiste; Mikoshiba, Katsuhiko; Triller, Antoine.

    In: Neuron, Vol. 62, No. 5, 11.06.2009, p. 670-682.

    Research output: Contribution to journalArticle

    Bannai, H, Lévi, S, Schweizer, C, Inoue, T, Launey, T, Racine, V, Sibarita, JB, Mikoshiba, K & Triller, A 2009, 'Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics', Neuron, vol. 62, no. 5, pp. 670-682. https://doi.org/10.1016/j.neuron.2009.04.023
    Bannai, Hiroko ; Lévi, Sabine ; Schweizer, Claude ; Inoue, Takafumi ; Launey, Thomas ; Racine, Victor ; Sibarita, Jean Baptiste ; Mikoshiba, Katsuhiko ; Triller, Antoine. / Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics. In: Neuron. 2009 ; Vol. 62, No. 5. pp. 670-682.
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