Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution

Degradation of HbV and erythropoiesis in a rat spleen for 2 weeks

Hiromi Sakai, Hirohisa Horinouchi, Manabu Yamamoto, Eiji Ikeda, Shinji Takeoka, Masuhiko Takaori, Eishun Tsuchida, Koichi Kobayashi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

BACKGROUND: Hemoglobin-vesicles (HbVs; diameter, 251 ± 81 nm) are artificial O2 carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. STUDY DESIGN AND METHODS: Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. RESULTS: The reduced hematocrit (Hct) level (26%) for the HbV/rHSA and rHSA groups returned to its original level (43%) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 ± 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 ± 22; sRBC/rHSA, 63 ± 7; baseline, 21 ± 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited in slight quantities for both the HbV/rHSA and sRBC/rHSA groups. Considerable amounts of erythroblasts were apparent in the spleens of both the rHSA and the HbV/rHSA groups. CONCLUSION: HbVs were phagocytized and degraded in RES, a physiological compartment for the degradation of RBCs, and the elevated erythropoietic activity resulted in the complete recovery of Hct within 7 days in the rat model.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalTransfusion
Volume46
Issue number3
DOIs
Publication statusPublished - 2006 Mar
Externally publishedYes

Fingerprint

Erythropoiesis
Serum Albumin
Hemoglobins
Spleen
Mononuclear Phagocyte System
Hematocrit
Hemosiderin
Erythroblasts
Splenomegaly
Hematopoiesis
Erythropoietin
Wistar Rats
Animal Models

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution : Degradation of HbV and erythropoiesis in a rat spleen for 2 weeks. / Sakai, Hiromi; Horinouchi, Hirohisa; Yamamoto, Manabu; Ikeda, Eiji; Takeoka, Shinji; Takaori, Masuhiko; Tsuchida, Eishun; Kobayashi, Koichi.

In: Transfusion, Vol. 46, No. 3, 03.2006, p. 339-347.

Research output: Contribution to journalArticle

Sakai, Hiromi ; Horinouchi, Hirohisa ; Yamamoto, Manabu ; Ikeda, Eiji ; Takeoka, Shinji ; Takaori, Masuhiko ; Tsuchida, Eishun ; Kobayashi, Koichi. / Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution : Degradation of HbV and erythropoiesis in a rat spleen for 2 weeks. In: Transfusion. 2006 ; Vol. 46, No. 3. pp. 339-347.
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title = "Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution: Degradation of HbV and erythropoiesis in a rat spleen for 2 weeks",
abstract = "BACKGROUND: Hemoglobin-vesicles (HbVs; diameter, 251 ± 81 nm) are artificial O2 carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. STUDY DESIGN AND METHODS: Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. RESULTS: The reduced hematocrit (Hct) level (26{\%}) for the HbV/rHSA and rHSA groups returned to its original level (43{\%}) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 ± 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 ± 22; sRBC/rHSA, 63 ± 7; baseline, 21 ± 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited in slight quantities for both the HbV/rHSA and sRBC/rHSA groups. Considerable amounts of erythroblasts were apparent in the spleens of both the rHSA and the HbV/rHSA groups. CONCLUSION: HbVs were phagocytized and degraded in RES, a physiological compartment for the degradation of RBCs, and the elevated erythropoietic activity resulted in the complete recovery of Hct within 7 days in the rat model.",
author = "Hiromi Sakai and Hirohisa Horinouchi and Manabu Yamamoto and Eiji Ikeda and Shinji Takeoka and Masuhiko Takaori and Eishun Tsuchida and Koichi Kobayashi",
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T1 - Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution

T2 - Degradation of HbV and erythropoiesis in a rat spleen for 2 weeks

AU - Sakai, Hiromi

AU - Horinouchi, Hirohisa

AU - Yamamoto, Manabu

AU - Ikeda, Eiji

AU - Takeoka, Shinji

AU - Takaori, Masuhiko

AU - Tsuchida, Eishun

AU - Kobayashi, Koichi

PY - 2006/3

Y1 - 2006/3

N2 - BACKGROUND: Hemoglobin-vesicles (HbVs; diameter, 251 ± 81 nm) are artificial O2 carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. STUDY DESIGN AND METHODS: Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. RESULTS: The reduced hematocrit (Hct) level (26%) for the HbV/rHSA and rHSA groups returned to its original level (43%) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 ± 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 ± 22; sRBC/rHSA, 63 ± 7; baseline, 21 ± 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited in slight quantities for both the HbV/rHSA and sRBC/rHSA groups. Considerable amounts of erythroblasts were apparent in the spleens of both the rHSA and the HbV/rHSA groups. CONCLUSION: HbVs were phagocytized and degraded in RES, a physiological compartment for the degradation of RBCs, and the elevated erythropoietic activity resulted in the complete recovery of Hct within 7 days in the rat model.

AB - BACKGROUND: Hemoglobin-vesicles (HbVs; diameter, 251 ± 81 nm) are artificial O2 carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. STUDY DESIGN AND METHODS: Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. RESULTS: The reduced hematocrit (Hct) level (26%) for the HbV/rHSA and rHSA groups returned to its original level (43%) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 ± 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 ± 22; sRBC/rHSA, 63 ± 7; baseline, 21 ± 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited in slight quantities for both the HbV/rHSA and sRBC/rHSA groups. Considerable amounts of erythroblasts were apparent in the spleens of both the rHSA and the HbV/rHSA groups. CONCLUSION: HbVs were phagocytized and degraded in RES, a physiological compartment for the degradation of RBCs, and the elevated erythropoietic activity resulted in the complete recovery of Hct within 7 days in the rat model.

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