Acute exercise activates local bioactive androgen metabolism in skeletal muscle

Katsuji Aizawa, Motoyuki Iemitsu, Seiji Maeda, Takeshi Otsuki, Koji Sato, Takashi Ushida, Noboru Mesaki, Takayuki Akimoto

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Androgens, such as testosterone, play important roles in regulation of diverse physiological process of target tissues. Recently, we reported that steroidogenic enzymes exist in skeletal muscle and regulate local production of testosterone in response to exercise. Testosterone is transformed into a bioactive androgen metabolite, dihydrotestosterone (DHT) by 5α-reductase. However, it is unclear whether exercise stimulates local bioactive androgen metabolism in the skeletal muscle in both sexes. In the present study, we examined sex differences in the levels of dehydroepiandrosterone (DHEA), free testosterone, DHT, and steroidogenesis-related enzymes 5α-reductase and androgen receptor (AR) in rat's skeletal muscle before and after a single bout of exercise. Basal muscular free testosterone and DHT levels were higher in males than females, whereas the levels of DHEA did not differ between the sexes. Muscular DHEA, free testosterone, and DHT levels were increased in both sexes after the exercise. There were no differences of 5α-reductase and AR transcripts and proteins between the sexes, and the expression of 5α-reductase was significantly increased in both sexes after the exercise. Finally, the expression of AR was significantly higher in female rats, but not in males after the exercise. These data suggest that acute exercise enhances the local bioactive androgen metabolism in the skeletal muscle of both sexes.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalSteroids
Volume75
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Keywords

  • 5α-reductase
  • Acute exercise
  • Androgen receptor
  • Dihydrotestosterone
  • Skeletal muscle
  • Testosterone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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  • Cite this

    Aizawa, K., Iemitsu, M., Maeda, S., Otsuki, T., Sato, K., Ushida, T., Mesaki, N., & Akimoto, T. (2010). Acute exercise activates local bioactive androgen metabolism in skeletal muscle. Steroids, 75(3), 219-223. https://doi.org/10.1016/j.steroids.2009.12.002