Acute stress response modified by modest inhibition of growth hormone axis: A potential machinery of the anti-aging effect of calorie restriction

Toshimitsu Komatsu, Lucas S. Trindade, Takuya Chiba, Hiroko Hayashi, Tomoko Henmi, Yoko Ushiroda, Ryoichi Mori, Isao Shimokawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Calorie restriction (CR) may exert antiaging effects by inhibiting the growth hormone (GH)/IGF-1 axis. The present study investigated the effect of modest inhibition of GH signaling on stress response and compared it with the effect of CR. Heterozygous (tg/-) rats of a transgenic strain of male rats, whose GH signaling was inhibited by overexpression of the anti-sense GH gene, and wild-type (WT) rats were used. Rats were fed ad libitum (AL) or 30% CR diets from 6 weeks of age. At 6 months of age, rats were killed between 0 and 8. h after lipopolysaccharide (LPS) injection to evaluate the acute phase stress response. tg/- rats had less tissue injury, indicated by blood aspartate aminotransferase (AST) concentrations, than WT rats. Successive waves of incremental plasma TNF-α, IL-6, and interferon (IFN)-γ levels were also attenuated in tg/- rats. Activation of NF-κB, a redox-sensitive transcription factor, was slightly diminished in tg/- rats, whereas the AP-1 activity was increased. Similar trends were also observed in the CR groups as compared to the AL groups. The present results suggest an involvement of the GH/IGF-1 axis in the effect of CR for stress response, even if CR does not act solely through the GH axis.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalMechanisms of Ageing and Development
Volume132
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Keywords

  • Calorie restriction
  • Endotoxin
  • Growth hormone
  • Inflammation

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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