Adaptation of macrophages to exercise training improves innate immunity

Takako Kizaki*, Tohru Takemasa, Takuya Sakurai, Tetsuya Izawa, Tomoko Hanawa, Shigeru Kamiya, Shukoh Haga, Kazuhiko Imaizumi, Hideki Ohno

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)


    The effects of 3-week exercise training on the functions of peritoneal macrophages from BALB/c mice were investigated. Lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and proinflammatory cytokine production in macrophages from trained mice was markedly higher than those from control mice. Meanwhile, exercise training decreased the steady state level of β2-adrenergic receptor (β2AR) mRNA in macrophages. Overexpression of β2AR in the macrophage cell line RAW264 by transfecting with β2AR cDNA suppressed NO synthase (NOS) II expression but dose not influenced proinflammatory cytokine expression. When expression of transfected β2AR in RAWar cells was downregulated by a tetracycline repressor-regulated mammalian expression system, NOS II mRNA expression was significantly increased; this suggested that the changes in the β2AR expression level in macrophages associated with exercise training play a role in the regulation of NO production following LPS stimulation. These findings indicate that exercise training improves macrophage innate immune function in a β2AR-dependent and -independent manner.

    Original languageEnglish
    Pages (from-to)152-156
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Issue number1
    Publication statusPublished - 2008 Jul 18


    • β-Adrenergic receptor
    • Exercise training
    • Innate immunity
    • Lipopolysaccharide
    • Macrophage
    • Nitric oxide synthase

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology


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