Adaptive Liquid Interfacially Assembled Protein Nanosheets for Guiding Mesenchymal Stem Cell Fate

Xiaofang Jia, Kosuke Minami, Koichiro Uto, Alice Chinghsuan Chang, Jonathan P. Hill, Jun Nakanishi, Katsuhiko Ariga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

There is a growing interest in the development of dynamic adaptive biomaterials for regulation of cellular functions. However, existing materials are limited to two-state switching of the presentation and removal of cell-adhesive bioactive motifs that cannot emulate the native extracellular matrix (ECM) in vivo with continuously adjustable characteristics. Here, tunable adaptive materials composed of a protein monolayer assembled at a liquid–liquid interface are demonstrated, which adapt dynamically to cell traction forces. An ultrastructure transition from protein monolayer to hierarchical fiber occurs through interfacial jamming. Elongated fibronectin fibers promote formation of elongated focal adhesion structures, increase focal adhesion kinase activation, and enhance neuronal differentiation of stem cells. Cell traction force results in spatial rearrangement of ECM proteins, which feeds back to alter stem cell fate. The reported biomimetic adaptive liquid interface enables dynamic control of stem cell behavior and has potential translational applications.

Original languageEnglish
Article number1905942
JournalAdvanced Materials
Volume32
Issue number4
DOIs
Publication statusPublished - 2020 Jan 1
Externally publishedYes

Keywords

  • adaptive materials
  • liquid–liquid interfaces
  • self-assembly
  • stem cell differentiation
  • stem cells

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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