Addition of short guanylyl blocks to oligonucleotide primers with a thermophilic polynucleotide phosphorylase: Its application to the synthesis of oligonucleotides containing guanylyl residues

Yo Kikuchi, Keiko Hirai, Kenji Sakaguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Polynucleotide phosphorylase from Thermus thermophilus catalyzed the addition of short guanylyl blocks from GDP to the 3'-hydroxyI termini of oligonucleotide primers at low temperature in a simple reaction mixture. Polyguanylic acid formation was inhibited at 37°C, but the addition of one or two guanylyl residues to oligonucleotide primers proceeded in high yields. The reaction was applied to the synthesis of oligonucleotides containing guanylyl residues at the 3'-end. Using (Ap)2A and (Up)2U as primers, (Ap)3G, (Ap)3GpG, and (Up)2G were synthesized in yields of 25-52%. (Ap)2GpG was synthesized from ApA and GDP in a yield of 13%.

Original languageEnglish
Pages (from-to)1427-1432
Number of pages6
JournalJournal of Biochemistry
Volume86
Issue number5
Publication statusPublished - 1979 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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